PKC mu Antibody (S05-4J5)

Western Blot: PKC mu Antibody (S05-4J5) [NBP3-14964] - Western blot detection of K562, C6, Hela cell lysates using NBP3-14964. Predicted band size: 102kDa. Observed band size: 102kDa.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: S05-4J5
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

PKC mu Antibody (S05-4J5) Summary

• Description: Novus Biologicals Rabbit PKC mu Antibody (S05-4J5) (NBP3-14964) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human PKC mu (Uniprot#: Q15139)
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PRKD1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000-1:2000
• Theoretical MW: 102 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS (pH 7.4), 150mM sodium chloride, 0.05% BSA, 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for PKC mu Antibody (S05-4J5)

• EC 2.7.11
• nPKC-D1
• nPKC-mu
• PKCM
• PKC-MU
• PKD1
• PKDEC 2.7.11.13
• PRKCMPKC-mu
• PRKD1
• Protein kinase C mu type
• protein kinase C, mu
• Protein kinase D
• protein kinase D1
• serine/threonine-protein kinase D1

Background

PKC mu is a novel member of the subgroup of atypical protein kinase Cs (PKC). Deduced protein sequence shows strong homology to conserved domains of members of the PKC subfamily. In vitro phorbol ester binding studies and kinase assays with lysates of cells overexpressing PKC mu showed phorbol ester-independent kinase activity, autophosphorylation, and, in normal rat kidney (NRK) cells, predominant phosphorylation of a 30-kDa protein at serine residues. Data suggest a role of PKC mu in signal transduction pathways related to growth control (1). PKC mu is a cytosolic protein, which upon binding to the trans-Golgi network (TGN) regulates the fission of transport carriers specifically destined to the cell surface. (2). Results demonstrate that betagamma subunits of the heterotrimeric G proteins directly activates PKD by interacting with its pleckstrin homology domain (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRKD1