Immunocytochemistry/ Immunofluorescence: PKHD1 Antibody [BC110-60480] - analysis of PKHD1 in mouse inner medullary collecting duct cell line using anti-PKHD1 antibody. Image from verified customer review.
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
PBS and 30% Glycerol
0.05% Sodium Azide
Immunogen affinity purified
Peptide ELISA 1:100-1:2000
This PKHD1 antibody is useful for peptide ELISA.
Read 1 Review rated 3 using BC110-60480 in the following application:
Human. Immunogen sequence has 86% identity to mouse.
Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at email@example.com for more details.
Alternate Names for PKHD1 Antibody
polycystic kidney and hepatic disease 1 (autosomal recessive)
PKHD1 protein (polycystic kidney and hepatic disease 1 protein) is encoded by PKHD1 gene whose mutations have been established as underlying cause of autosomal recessive polycystic kidney disease (ARPKD), a rare genetic disorder characterized mainly by cystic kidneys in the developing fetus in utero and post-natal pulmonary insufficiency as well as renal manifestations, abnormal biliary development with dilated bile ductules and peribiliary fibrosis, collectively termed congenital hepatic fibrosis (CHF). PKHD1 is a type I membrane protein with a large extracellular N-terminal domain, a single transmembrane segment, and a short cytoplasmic C-terminus. PKHD1 is a large protein with predicted molecular weight of 447 kD and is predominantly expressed in kidneys (the cortical and medullary collecting ducts), pancreas, liver etc. and in addition to other locations in the cells, it is localized on primary cilium, hair-like organelle present on cellular surfaces. PKHD1 is essential for correct bipolar cell division through the regulation of centrosome duplication and mitotic spindle assembly. Moreover, it has been suggested to act as a receptor that plays a role in collecting duct/biliary differentiation and lack of PKHD1 results in stunted primary cilia which have been proposed to underlie the pathogenesis of ARPKD.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.