PKC beta Antibody

Images

 
Western Blot: PKC beta Antibody [NBP2-19846] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC beta antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: PKC beta Antibody [NBP2-19846] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: PKC beta Antibody [NBP2-19846] - Paraffin-embedded rat brain. PKC beta antibody diluted at 1:500.
Western Blot: PKC beta Antibody [NBP2-19846] - Sample (30 ug of whole cell lysate) A: IMR32 7. 5% SDS PAGE gel, diluted at 1:1000.
Western Blot: PKC beta Antibody [NBP2-19846] - Human neuroblastoma cells untreated or treated with various drugs. Image from verified customer review.
Immunohistochemistry-Paraffin: PKC beta Antibody [NBP2-19846] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:250 dilution.

Product Details

Summary
Reactivity Hu, Rt, Bovine, Mouse, Rabbit, MonkeySpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Concentration
0.63 mg/ml

Order Details

PKC beta Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human PKC beta. The exact sequence is proprietary.
Localization
Cytoplasm (By similarity); Membrane; Peripheral membrane protein
Predicted Species
Mouse (98%), Monkey (100%), Rabbit (98%), Bovine (97%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PRKCB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.63 mg/ml
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
Application Notes
IHC-P usage reported in scientific literature (PMID: 25135933). The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
77 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Rhesus Monkey (100%).

Alternate Names for PKC beta Antibody

  • EC 2.7.11
  • EC 2.7.11.13
  • MGC41878
  • PKC-B
  • PKC-beta
  • PKCBPRKCB2
  • PRKCB1protein kinase C beta 1
  • protein kinase C beta type
  • protein kinase C, beta 1 polypeptide
  • protein kinase C, beta 1
  • protein kinase C, beta

Background

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol PRKCB
Uniprot

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