PKC epsilon Antibody (OTI4G8)

Western Blot: PKC epsilon Antibody (4G8) [NBP2-03620] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-PKC epsilon monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunocytochemistry/Immunofluorescence: PKC epsilon Antibody (4G8) [NBP2-03620] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PKC epsilon.

Immunohistochemistry-Paraffin: PKC epsilon Antibody (4G8) [NBP2-03620] - Staining of paraffin-embedded Human prostate tissue using anti-PKC epsilon mouse monoclonal antibody.

Flow Cytometry: PKC epsilon Antibody (4G8) [NBP2-03620] - Analysis of Hela cells, using anti-PKC epsilon antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Western Blot: PKC epsilon Antibody (4G8) [NBP2-03620] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PKC epsilon (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PKC epsilon.

Immunohistochemistry-Paraffin: PKC epsilon Antibody (4G8) [NBP2-03620] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PKC epsilon mouse monoclonal antibody.

Immunohistochemistry-Paraffin: PKC epsilon Antibody (4G8) [NBP2-03620] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PKC epsilon mouse monoclonal antibody.

Immunohistochemistry-Paraffin: PKC epsilon Antibody (4G8) [NBP2-03620] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PKC epsilon mouse monoclonal antibody.

Immunohistochemistry-Paraffin: PKC epsilon Antibody (4G8) [NBP2-03620] - Staining of paraffin-embedded Human lymph node tissue using anti-PKC epsilon mouse monoclonal antibody.

Immunohistochemistry-Paraffin: PKC epsilon Antibody (4G8) [NBP2-03620] - Staining of paraffin-embedded Human lymphoma tissue using anti-PKC epsilon mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt, Pm, Pm
• Applications: WB, Flow, ICC/IF, IHC
• Clone: OTI4G8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PKC epsilon Antibody (OTI4G8) Summary

• Description: Novus Biologicals Mouse PKC epsilon Antibody (OTI4G8) (NBP2-03620) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human PRKCE (NP_005391) produced in HEK293T cell.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PRKCE
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:1000-2000
• Theoretical MW: 83.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PKC epsilon Antibody (OTI4G8)

• EC 2.7.11
• EC 2.7.11.13
• MGC125656
• nPKC-epsilon
• PKC epsilon
• PKCE
• PKCEMGC125657
• PRKCE
• protein kinase C epsilon type
• protein kinase C, epsilon

Background

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may also play a role in controlling anxiety-like behavior. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRKCE
• Entrez:
  • 5581(Human)