PKC beta Antibody - BSA Free

Western Blot: PKC beta Antibody [NB110-86972] - Analysis of PKC beta in 25 g of U251 whole cell lysate (glioma derived). A 4-12% gel was used for separation. The arrowhead corresponds to 76 kDa PKC beta. The membrane was probed with the primary antibody at a 1:1,000 dilution in 5% BSA in TTBS at 4 C, overnight.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PKC beta Antibody - BSA Free Summary

• Description: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: This affinity purified PKC beta Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human PKC beta. (Uniprot: P05771)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRKCB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:75000-1:80000
  • Western Blot 1:1000-1:2000
• Application Notes: This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 76 kDa in size corresponding to PKC beta by western blotting in the appropriate cell lysate or extract. Lysates from U251 or BxPC3 cells are suggested for western blotting.

Reactivity Notes

A BLAST analysis was used to suggest cross-reactivity with PKC beta from human and monkey sources based on a 100% homology with the immunizing sequence. Expect partial cross reactivity to PKC beta from rabbit and bovine (93% homology) and rat and mouse (87% homology) sources. Cross-reactivity with PKC beta from other sources has not been determined.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for PKC beta Antibody - BSA Free

• EC 2.7.11
• EC 2.7.11.13
• MGC41878
• PKC-B
• PKC-beta
• PKCBPRKCB2
• PRKCB1protein kinase C beta 1
• protein kinase C beta type
• protein kinase C, beta 1 polypeptide
• protein kinase C, beta 1
• protein kinase C, beta

Background

Protein kinase C (PKC) beta is implicated as a potential important factor in cancer cell proliferation and angiogenesis. PKC is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRKCB
• Entrez:
  • 5579(Human)
  • 18751(Mouse)
  • 293508(Rat)
• Uniprot:
  • P05771()
  • P05771()
  • P05771()
  • P05771()
  • P05771()