PKA 2 beta Antibody - BSA Free

Images

 
Western Blot: PKA 2 beta Antibody [NBP1-33773] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PKA 2 beta antibody diluted at 1:1000. The HRP-conjugated anti-rabbit ...read more
Immunocytochemistry/ Immunofluorescence: PKA 2 beta Antibody [NBP1-33773] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Frozen: PKA 2 beta Antibody [NBP1-33773] - Frozen Sectioned adult mouse hippocampus. Green: PKA 2 beta protein stained by PKA 2 beta antibody diluted at 1:250. Blue: Fluoroshield with DAPI. Antigen ...read more
Western Blot: PKA 2 beta Antibody [NBP1-33773] - Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 10% SDS PAGE; antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: PKA 2 beta Antibody [NBP1-33773] - Human colon carcinoma, using PKA 2 beta antibody at 1:500 dilution.Antigen Retrieval: Citrate buffer, pH 6.0, 10 min.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free

Order Details

PKA 2 beta Antibody - BSA Free Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human PKA 2 beta. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PRKAR2B
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus laevis (84%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for PKA 2 beta Antibody - BSA Free

  • cAMP-dependent protein kinase type II-beta regulatory chain
  • cAMP-dependent protein kinase type II-beta regulatory subunit
  • H_RG363E19.2
  • PKA 2 beta
  • PRKAR2
  • PRKAR2B
  • protein kinase, cAMP-dependent, regulatory, type II, beta
  • RII-BETA
  • WUGSC:H_RG363E19.2

Background

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. This subunit has been shown to interact with and suppress the transcriptional activity of the cAMP responsive element binding protein 1 (CREB1) in activated T cells. Knockout studies in mice suggest that this subunit may play an important role in regulating energy balance and adiposity. The studies also suggest that this subunit may mediate the gene induction and cataleptic behavior induced by haloperidol.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PRKAR2B