PKA 2 beta Antibody

Images

 
Western Blot: PKA 2 beta Antibody [NBP1-33773] - Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 10% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: PKA 2 beta Antibody [NBP1-33773] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: PKA 2 beta Antibody [NBP1-33773] - Paraffin-embedded Colon ca, using antibody at 1:500 dilution.
Western Blot: PKA 2 beta Antibody [NBP1-33773] - Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE; antibody diluted at 1:1000.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.67 mg/ml

Order Details

PKA 2 beta Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human PKA 2 beta. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PRKAR2B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Xenopus laevis (84%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.67 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PKA 2 beta Antibody

  • cAMP-dependent protein kinase type II-beta regulatory chain
  • cAMP-dependent protein kinase type II-beta regulatory subunit
  • H_RG363E19.2
  • PRKAR2
  • protein kinase, cAMP-dependent, regulatory, type II, beta
  • RII-BETA
  • WUGSC:H_RG363E19.2

Background

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. This subunit has been shown to interact with and suppress the transcriptional activity of the cAMP responsive element binding protein 1 (CREB1) in activated T cells. Knockout studies in mice suggest that this subunit may play an important role in regulating energy balance and adiposity. The studies also suggest that this subunit may mediate the gene induction and cataleptic behavior induced by haloperidol.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-47935
Species: Hu, Mk
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-02520
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP1-89172
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-00634
Species: Hu, Mk
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-38713
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-50037
Species: Hu, Mu, Rt, Po, Bv, Eq
Applications: WB, ICC/IF
NB100-56749
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP1-30475
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP, PLA
AF682
Species: Hu
Applications: WB, IHC, ELISA(Cap), ELISA(Det), Neut, ELISA(Sta)
NB100-105
Species: Hu, Mu, Rt, Po, Bv, Ft, Mk, Pm, Rb, Sh, Xp, Ye
Applications: WB, ChIP, ELISA, Flow, GS, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, TCS, KO, LA
NB100-410
Species: Hu, Mu
Applications: WB, IP
PP-N1665-00
Species: Hu
Applications: WB, IHC, IP
NB110-37253
Species: Hu, Mu, Rt
Applications: WB
MAB4500
Species: Hu
Applications: CyTOF-ready, ICFlow

Publications for PKA 2 beta Antibody (NBP1-33773) (0)

There are no publications for PKA 2 beta Antibody (NBP1-33773).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for PKA 2 beta Antibody (NBP1-33773) (0)

There are no reviews for PKA 2 beta Antibody (NBP1-33773). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PKA 2 beta Antibody (NBP1-33773) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional PKA 2 beta Products

Bioinformatics Tool for PKA 2 beta Antibody (NBP1-33773)

Discover related pathways, diseases and genes to PKA 2 beta Antibody (NBP1-33773). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for PKA 2 beta Antibody (NBP1-33773)

Discover more about diseases related to PKA 2 beta Antibody (NBP1-33773).
 

Pathways for PKA 2 beta Antibody (NBP1-33773)

View related products by pathway.

PTMs for PKA 2 beta Antibody (NBP1-33773)

Learn more about PTMs related to PKA 2 beta Antibody (NBP1-33773).
 

Research Areas for PKA 2 beta Antibody (NBP1-33773)

Find related products by research area.

Blogs on PKA 2 beta

There are no specific blogs for PKA 2 beta, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our PKA 2 beta Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol PRKAR2B