PIST Antibody

Images

 
Western Blot: PIST Antibody [NBP1-77242] - Analysis in rat colon cell lysate with PIST antibody at (A) 1, (B) 2 and (C) 4 ug/mL.
Immunohistochemistry: PIST Antibody [NBP1-77242] - Staining of PIST in human testis tissue with PIST antibody at 2 ug/ml.
Immunohistochemistry-Paraffin: PIST Antibody [NBP1-77242] - Immunohistochemistry of PIST in rat colon tissue with PIST antibody at 1 ug/mL.
Immunohistochemistry: PIST Antibody [NBP1-77242] - Staining of PIST in human testis tissue with PIST antibody at 20 ug/ml.
Immunofluorescence: PIST Antibody [NBP1-77242] - Immunofluorescence of PIST in Rat Colon cells with PIST antibody at 10 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, IHC, IHC-P, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

PIST Antibody Summary

Immunogen
Antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human PIST. The immunogen is located within amino acids 360 - 410 of PIST.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GOPC
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunofluorescence 10 ug/ml
Application Notes
PIST antibody can be used for the detection of PIST by Western blot at 1 - 2 mg/ml.
Control Peptide
PIST Peptide (NBP1-77242PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PIST Antibody

  • CALPDZ protein interacting specifically with TC10
  • CFTR-associated ligand
  • dJ94G16.2
  • FIGdJ94G16.2 PIST
  • Fused in glioblastoma
  • golgi-associated PDZ and coiled-coil motif containing
  • Golgi-associated PDZ and coiled-coil motif-containing protein
  • GOPC1
  • PDZ/coiled-coil domain binding partner for the rho-family GTPase TC10
  • PISTGolgi associated PDZ and coiled-coil motif containing protein

Background

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components and is negatively regulated by TOR (Target of rapamycin). PIST, a PDZ-containing protein, was discovered in a yeast two-hybrid system as a binding partner to Beclin-1, a Bcl-2-interacting protein homologous to the yeast autophagy gene apg6. Experiments with mutant PIST proteins lacking the PDZ domain showed that PIST interaction with Beclin-1 through its coiled-coil domain can modulate Beclin-1 activity and suggest that PIST interactions with other proteins through its PDZ domain may regulate the activity of PIST and Beclin-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol GOPC
Uniprot