PIM1 Antibody

Images

 
Western Blot: PIM1 Antibody [NBP2-31366] - Western blot analysis of HeLa (A) and A431 (B) cell lysate using PIM1 antibody at a concentration of 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: PIM1 Antibody [NBP2-31366] - PIM1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). ...read more
Immunohistochemistry-Paraffin: PIM1 Antibody [NBP2-31366] - IHC-P analysis of PIM1 protein in a section of human esophageal squamous cell carcinoma (SCC) using PIM1 antibody at a concentration of 5 ug/ml. The ...read more
Immunohistochemistry-Paraffin: PIM1 Antibody [NBP2-31366] - IHC-P analysis of PIM1 protein in a section of human breast normal tissue using PIM1 antibody at a concentration of 5 ug/ml. The breast ductal or acinar ...read more

Product Details

Summary
Reactivity Hu, Rt, Bv, FeSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

PIM1 Antibody Summary

Immunogen
Partial recombinant protein made to an N-terminal portion of human PIM1 (between residues 1-200) [UniProt# P11309]
Localization
Cytoplasm, Nucleus, Cell Membrane
Specificity
This antibody may detect isoform 1 as well as isoform 2 of PIM1 (Uniprot # P11309).
Predicted Species
Rat (97%), Bovine (97%), Feline (97%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PIM1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:100
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
45.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human. Based upon 97% similarity to immunogen sequence, this antibody is predicted to react with Rat, Bovine and Feline (Cat). For Mouse's PIM1, the immunogen sequence shows 97% similarity to isoform 2 and 74% similarity to isoform 1.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for PIM1 Antibody

  • EC 2.7.11.1
  • Oncogene PIM1
  • PIM
  • pim-1 kinase 44 kDa isoform
  • pim-1 oncogene (proviral integration site 1)
  • pim-1 oncogene
  • PIM1
  • proto-oncogene serine/threonine-protein kinase pim-1

Background

PIM1 belongs to the Ser/Thr protein kinase family, and PIM subfamily. This protein is expressed primarily in B-lymphoid and myeloid cell lines, and is overexpressed in hematopoietic malignancies and in prostate cancer. It plays a role in signal transduction in blood cells, contributing to both cell proliferation and survival, and thus provides a selective advantage in tumorigenesis. Both the human and orthologous mouse genes have been reported to encode two isoforms (with preferential cellular localization) resulting from the use of alternative in-frame translation initiation codons, the upstream non-AUG (CUG) and downstream AUG codons. Recombinant human Pim1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PIM1 Antibody (NBP2-31366) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PIM1
Entrez
Uniprot