PIK3CA Antibody

Images

 
Immunohistochemistry-Paraffin: PIK3CA Antibody [NB100-55408] - Staining of paraffin embedded human heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: PIK3CA Antibody [NB100-55408] - Staining of paraffin embedded human brain (cerebellar cortex). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

PIK3CA Antibody Summary

Immunogen
Peptide with sequence C-HQWLKDKNKGEIYD corresponding to internal region according to NP_006209.2.
Epitope
HQWLKDKNKGEIYD
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PIK3CA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • Immunohistochemistry 2 - 5 ug/ml
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Peptide ELISA Detection limit 1:128000
Application Notes
WB: Preliminary experiments in lysates of A431, HeLa and Jurkat gave no specific signal but low background. IHC-P: Human brain (cerebral cortex) shows cytoplasm stained in the cell bodies. IHC successful usage on human epithelial ovarian carcinoma is reported in scientific literature (PMID: 19638206).
Positive Control
PIK3CA Lysate (NBL1-14417)
Publications
Read Publications using NB100-55408.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PIK3CA Antibody

  • EC 2.7.1
  • EC 2.7.1.153
  • MGC142161
  • MGC142163
  • p110-alpha
  • Phosphatidylinositol 3-Kinase catalytic subunit alpha
  • phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha
  • phosphatidylinositol 3-kinase, catalytic, alpha polypeptide
  • Phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha
  • phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
  • phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit, alpha isoform
  • phosphoinositide-3-kinase, catalytic, alpha polypeptide
  • PI3K
  • PI3K-alpha
  • PI3-kinase p110 subunit alpha
  • PI3-kinase subunit alpha
  • PtdIns-3-kinase p110
  • PtdIns-3-kinase subunit alpha
  • PtdIns-3-kinase subunit p110-alpha

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PIK3CA Antibody (NB100-55408)(2)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for PIK3CA Antibody (NB100-55408) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PIK3CA
Entrez
Uniprot