PIK3CA Antibody

Immunohistochemistry-Paraffin: PIK3CA Antibody [NB100-55408] - Staining of paraffin embedded human heart with antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: PIK3CA Antibody [NB100-55408] - Staining of paraffin embedded human brain (cerebellar cortex) with antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PIK3CA Antibody Summary

• Description: Novus Biologicals Goat PIK3CA Antibody (NB100-55408) is a polyclonal antibody validated for use in IHC and ELISA. Anti-PIK3CA Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-HQWLKDKNKGEIYD corresponding to internal region according to NP_006209.2.
• Epitope: HQWLKDKNKGEIYD
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PIK3CA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 2 - 3 ug/mL
  • Immunohistochemistry-Paraffin 2-3 ug/mL
  • Peptide ELISA Detection limit 1:128000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PIK3CA Antibody

• CLAPO
• CLOVE
• CWS5
• EC 2.7.1
• EC 2.7.1.153
• MCMTC
• MGC142161
• MGC142163
• P110alpha
• p110-alpha
• Phosphatidylinositol 3-Kinase catalytic subunit alpha
• phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha
• phosphatidylinositol 3-kinase, catalytic, alpha polypeptide
• Phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha
• phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
• phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit, alpha isoform
• phosphoinositide-3-kinase, catalytic, alpha polypeptide
• PI3K
• PI3Kalpha
• PI3K-alpha
• PI3-kinase p110 subunit alpha
• PI3-kinase subunit alpha
• PIK3CA
• PtdIns-3-kinase p110
• PtdIns-3-kinase subunit alpha
• PtdIns-3-kinase subunit p110-alpha

Background

PI3-Kinases (PI3-Ks) are a family of lipid kinases that are implicated in signal transduction. PI3-K consists of two subunits; p85 and p10. The p85 subunit localize PI3-K activity to the plasma membrane while the p110 subunit contains the catalytic domain of PI3-K (1-2). Four isoforms of p110 has been found;

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PIK3CA Antibody (NB100-55408)(2)

Publications using NB100-55408

• Abubaker J, Bavi P, Al-Haqawi W et al. PIK3CA alterations in Middle Eastern ovarian cancers. Mol Cancer 2009-07-01 [PMID: 19638206]
• Lopez-Knowles E, Hernandez S, Malats N et al. PIK3CA mutations are an early genetic alteration associated with FGFR3 mutations in superficial papillary bladder tumors. Cancer Res 2006-08-01 [PMID: 16885334]

Bioinformatics

• Gene Symbol: PIK3CA
• Entrez:
  • 5290(Human)
  • 18706(Mouse)
• Uniprot:
  • NP_006209.2()