PIK3CA Antibody (3G3) - Azide and BSA Free

Proximity Ligation Assay: PIK3CA Antibody (3G3) [H00005290-M01] - Analysis of protein-protein interactions between APPL1 and PIK3CA. HeLa cells were stained with anti-APPL1 rabbit purified polyclonal 1:1200 and anti-PIK3CA mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Sandwich ELISA: PIK3CA Antibody (3G3) [H00005290-M01] - Detection limit for recombinant GST tagged PIK3CA is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: ELISA, Func
• Clone: 3G3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PIK3CA Antibody (3G3) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: PIK3CA (NP_006209, 959 a.a. ~ 1068 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DFLIVISKGAQECTKTREFERFQEMCYKAYLAIRQHANLFINLFSMMLGSGMPELQSFDDIAYIRKTLALDKTEQEALEYFMKQMNDAHHGGWTTKMDWIFHTIKQHALN
• Specificity: PIK3CA (3G3)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PIK3CA
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Proximity Ligation Assay
  • Sandwich ELISA
• Application Notes: Antibody reactivity against recombinant protein on ELISA. GST alone used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PIK3CA Antibody (3G3) - Azide and BSA Free

• CLAPO
• CLOVE
• CWS5
• EC 2.7.1
• EC 2.7.1.153
• MCMTC
• MGC142161
• MGC142163
• P110alpha
• p110-alpha
• Phosphatidylinositol 3-Kinase catalytic subunit alpha
• phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha
• phosphatidylinositol 3-kinase, catalytic, alpha polypeptide
• Phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha
• phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
• phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit, alpha isoform
• phosphoinositide-3-kinase, catalytic, alpha polypeptide
• PI3K
• PI3Kalpha
• PI3K-alpha
• PI3-kinase p110 subunit alpha
• PI3-kinase subunit alpha
• PIK3CA
• PtdIns-3-kinase p110
• PtdIns-3-kinase subunit alpha
• PtdIns-3-kinase subunit p110-alpha

Background

Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PIK3CA
• Entrez:
  • 5290(Human)
• OMIM:
  • 114480(Human)
• Uniprot:
  • NP_006209(Human)