PIEZO1 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: PIEZO1 Antibody [NBP1-78446] - PIEZO1 antibody (Goat anti-rabbit 568) was tested in MDCK cells. Nuclei and B-actin were counterstained with DAPI (blue) and phalloidin (green). Image from verified customer review.

Immunohistochemistry: PIEZO1 Antibody [NBP1-78446] - Analysis of PIEZ01 in mouse epidermis using DAB with hematoxylin counterstain.

Flow Cytometry: PIEZO1 Antibody [NBP1-78446] - An intracellular stain was performed on MCF7 cells with PIEZO1 Antibody NBP1-78446 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

Immunocytochemistry/Immunofluorescence: PIEZO1 Antibody [NBP1-78446] - PIEZO1 antibody was tested in A431 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

PIEZO1 was detected in immersion fixed MCF7 human breast cancer cell line using Rabbit anti-PIEZO1 Affinity Purified Polyclonal Antibody conjugated to DyLight 650 (Catalog # NBP1-78446C) (light blue) at 10 µg/mL overnight at 4C. Cells were stained counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

PIEZO1 was detected in immersion fixed MCF7 human breast cancer cell line using Rabbit anti-PIEZO1 Affinity Purified Polyclonal Antibody conjugated to Janelia Fluor® 549 (Catalog # NBP1-78446JF549) (red) at 10 µg/mL overnight at 4C. Cells were stained counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

• Reactivity: Hu, Mu, Gp
• Applications: WB, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

PIEZO1 Antibody - BSA Free Summary

• Immunogen: A synthetic peptide made to an internal portion of the human PIEZO1 protein (between residues 950-1000) [UniProt Q92508]
• Localization: Endoplasmic reticulum membrane; Multi-pass membrane protein. Endoplasmic reticulum-Golgi intermediate compartment membrane. Cell membrane; Multi-pass membrane protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PIEZO1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow (Intracellular) 1 - 5 ug/ml
  • Flow Cytometry 1-5 ug/ml. Use reported in scientific literature (PMID 31685811)
  • Immunocytochemistry/ Immunofluorescence 1:25
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400
  • Western Blot 2 ug/ml
• Application Notes: According to Gudipaty et al "only this Piezo1 antibody reliably worked for Piezo1 immunostaining, as it vanishes in cells mosaically expressing a Piezo1-shRNA-mCherry construct" (PMID: 28199303). Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
• Control Peptide:

  PIEZO1 Peptide (NBP1-78446PEP)

Reactivity Notes

Guinea Pig reactivity reported in scientific literature (PMID: 30324494).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PIEZO1 Antibody - BSA Free

• FAM38A
• KIAA0233
• member A
• membrane protein induced by beta-amyloid treatment
• Mib
• PIEZO1
• piezo-type mechanosensitive ion channel component 1
• Protein FAM38A

Background

Piezo1 (Mib, FAM38A) is a mechanosensitive ion channel which responds to mechanically activated currents in numerous cells, including the brain, bladder, colon, kidney, lung, and skin. However, mRNA expression of Piezo1 is weak in the DRG, unlike Piezo2. The expression of Piezo1 is induced in Alzheimer's associated senile plaques (PMID 16854388), as well as integrin activation through R-Ras signaling (PMID 20016066). R-Ras is recruited to the endoplasmic reticulum which increases calcium release, leading to the activation of the protease calpain. Piezo proteins are fairly novel proteins, and whose sequences are not akin to other ion channels or proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PIEZO1 Antibody (NBP1-78446)(28)

Publications using NBP1-78446

• Zhao T, Chu Z, Chu C et al. Macrophages induce gingival destruction via Piezo1-mediated MMPs-degrading collagens in periodontitis Frontiers in Immunology 2023-05-16 [PMID: 37261355] (IHC-P, Human)
  
  Details:
  Dilution: 1:100
• Foote A, Tibbetts J, Bartley S, Thibeault S Localization of TRPV3/4 and PIEZO1/2 sensory receptors in murine and human larynges Laryngoscope Investigative Otolaryngology 2022-11-02 [PMID: 36544955] (IHC-P, Human)
  
  Details:
  Dilution used in IHC 1:200
• Greenlee J, Liu K, Lopez-Cavestany M, King M Piezo1 Mechano-Activation Is Augmented by Resveratrol and Differs between Colorectal Cancer Cells of Primary and Metastatic Origin Molecules 2022-08-25 [PMID: 36080197] (FLOW, Human)
• Mylvaganam S, Plumb J, Yusuf B et al. The spectrin cytoskeleton integrates endothelial mechanoresponses Nature cell biology 2022-07-11 [PMID: 35817960] (ICC/IF, WB, Human)
  
  Details:
  ICC/IF Dilution: 4.5 ug ml–1, WB Dilution: 1:1000
• Harraz OF, Klug NR, Mughal A Piezo1 channels are mechanosensors in brain capillaries Circ Res 2022-04-06 [PMID: 35382561]
• O'Callaghan P, Engberg A, Fatsis-Kavalopoulos N Piezo1 activation attenuates thrombin-induced blebbing in breast cancer cells J Cell Sci 2022-03-11 [PMID: 35274124]
• Tijore A, Yao M, Wang YH et al. Selective killing of transformed cells by mechanical stretch Biomaterials 2021-05-21 [PMID: 34044258]
• Passini FS, Jaeger PK, Saab AS et al. Shear-stress sensing by PIEZO1 regulates tendon stiffness in rodents and influences jumping performance in humans Nature biomedical engineering 2021-05-24 [PMID: 34031557] (ICC/IF, Human)
  
  Details:
  Immunocytochemistry was performed using the PIEZO1 antibody on human cells.
• Zhang G, Li X, Wu L, Qin YX Piezo1 channel activation in response to mechanobiological acoustic radiation force in osteoblastic cells Bone research 2021-03-10 [PMID: 33692342] (ICC/IF, WB)
• Silver BB, Wolf AE, Lee J et al. Epithelial tissue geometry directs emergence of bioelectric field and pattern of proliferation Mol. Biol. Cell 2020-06-10 [PMID: 32520653]
• dela Paz, N G & Frangos, J A. Rapid flow-induced activation of G alpha q/11 is independent of Piezo1 activation. Am J Physiol Cell Physiol 2019-05-01 [PMID: 30811222] (IHC-Fr, Mouse, Human)
• Passini F, Jaeger P, Saab A et al. Shear sensor mechano-signaling determines tendon stiffness and human jumping performance Research Square 2020-11-10 (ICC/IF, Human)
• MIKHAILOV N Dissertations in Health Sciences Thesis 2020-01-01 (IF/IHC, Mouse)
• Yao M, Tijore As, Cox Cd et Al. Force-dependent Piezo1 recruitment to focal adhesions regulates adhesion maturation and turnover specifically in non-transformed cells bioRxiv 2020-03-09 (IF/IHC, Human)
• Mao C, Yu W, Li G et Al. Effects of immediate loading directionality on the mechanical sensing protein PIEZO1 expression and early-stage healing process of peri-implant bone Biomed Eng Online 2024-03-19 [PMID: 38504231]
• Chang Liu, Yanan Xia, Shichen Fu, Fanyi Meng, Bingcheng Feng, Leiqi Xu, Lixiang Li, Xiuli Zuo Inhibition of Piezo1 Ameliorates Intestinal Inflammation and Limits the Activation of Group 3 Innate Lymphoid Cells in Experimental Colitis Journal of Innate Immunity 2023-09-19 [PMID: 37725937]
• Mitchell SJ, Pardo-Pastor C, Zangle TA, Rosenblatt J Voltage-dependent volume regulation controls epithelial cell extrusion and morphology bioRxiv : the preprint server for biology 2023-03-14 [PMID: 36993671] (ICC/IF, Canine)
• Singh A, Tijore A, Margadant F et al. Enhanced tumor cell killing by ultrasound after microtubule depolymerization Bioengineering & Translational Medicine 2021-09-01 [PMID: 34589605] (Knockdown Validated, Immunocytochemistry/ Immunofluorescence, Canine)
• Hope JM, Lopez-Cavestany M, Wang W et al. Activation of Piezo1 sensitizes cells to TRAIL-mediated apoptosis through mitochondrial outer membrane permeability Cell Death Dis 2019-11-04 [PMID: 31685811] (FLOW, Human)
• Harraz OF, Klug NR, Mughal A Piezo1 channels are mechanosensors in brain capillaries Poster (ICC/IF, Mouse)
• Fujie T, Takenaka F, Yoshida E et al. Shear stress induced nuclear shrinkage through activation of Piezo1 channels in epithelial cells J. Cell. Sci. 2019-05-10 [PMID: 31076516]
• Mazzuoli-Weber G, Kugler EM, Buhler CI et al. Piezo proteins: incidence and abundance in the enteric nervous system. Is there a link with mechanosensitivity. Cell Tissue Res. 2018-10-15 [PMID: 30324494] (IF/IHC, Guinea Pig)
• Gudipaty S, Lindblom J, Loftus P et al. Mechanical stretch triggers rapid epithelial cell division through Piezo1. Nature. 2017-03-02 [PMID: 28199303] (Knockdown Validated, Immunocytochemistry/ Immunofluorescence, Canine)
• Kotaro Hirano, Masaki Tsuchiya, Akifumi Shiomi, Seiji Takabayashi, Miki Suzuki, Yudai Ishikawa, Yuya Kawano, Yutaka Takabayashi, Kaori Nishikawa, Kohjiro Nagao, Eiji Umemoto, Yasuo Kitajima, Yusuke Ono, Keiko Nonomura, Hirofumi Shintaku, Yasuo Mori, Masato Umeda, Yuji Hara The mechanosensitive ion channel PIEZO1 promotes satellite cell function in muscle regeneration Life Science Alliance 2022-11-29 [PMID: 36446523]
• Yao M, Tijore A, Cheng D et al. Force- and cell state-dependent recruitment of Piezo1 drives focal adhesion dynamics and calcium entry Science advances 2022-11-11 [PMID: 36351022] (ICC/IF, Human)
• Y Mitsuhata, T Abe, K Misaki, Y Nakajima, K Kiriya, M Kawasaki, H Kiyonari, M Takeichi, M Toya, M Sato Cyst formation in proximal renal tubules caused by dysfunction of the microtubule minus-end regulator CAMSAP3 Scientific Reports, 2021-03-12;11(1):5857. 2021-03-12 [PMID: 33712686] (Knockdown Validated, Immunocytochemistry/ Immunofluorescence, Canine)
• Austin Lai, Yung C Chen, Charles D Cox, Anthony Jaworowski, Karlheinz Peter, Sara Baratchi Analyzing the shear-induced sensitization of mechanosensitive ion channel Piezo-1 in human aortic endothelial cells. Journal of cellular physiology 2021-09-21 [PMID: 32959903]

Review for PIEZO1 Antibody (NBP1-78446) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Canine.

reviewed by: Swapna A. Gudipaty ICC Canine 06/26/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: MDCK canine kidney epithelial cell line
• Species: Canine
• Lot: A-1

FAQs for PIEZO1 Antibody (NBP1-78446). (Showing 1 - 1 of 1 FAQs).

1. I am interested in purchasing the PIEZO 1 and PIEZO 2 antibodies and their blocking peptides from Novus. I have read your data sheets that in WBs the bands seen are approximately 286 kDa and 318 kDa, respectively. I would greatly appreciate if you could show me these WBs on human cells For PIEZO1 and 2 and the effects of the blocking peptides (or point me to publications using your Abs).
   • We offer a PIEZO1 blocking peptide specific to product NBP1-78446. We unfortunately do not offer a PIEZO2 blocking peptide. Our western blot data for NBP1-78446, NBP1-78624, and NBP1-78538 was performed by an outside collaborator and they have not given permission to us to release their images. That is why we do not have them available on our website. We will fully guarantee all of our products for any listed application or species. We unfortunately do not have any publications for these products either.

Bioinformatics

• Gene Symbol: PIEZO1
• Entrez:
  • 9780(Human)
• Uniprot:
  • Q92508(Human)