Immunocytochemistry: PIEZO1 Antibody [NBP1-78446] - PIEZO1 antibody (Goat anti-rabbit 568) was tested in MDCK cells. Nuclei and B-actin were counterstained with DAPI (blue) and phalloidin (green). Image from verified ...read more
Immunohistochemistry: PIEZO1 Antibody [NBP1-78446] - Analysis of PIEZ01 in mouse epidermis using DAB with hematoxylin counterstain.
Immunocytochemistry/ Immunofluorescence: PIEZO1 Antibody [NBP1-78446] - PIEZO1 antibody was tested in A431 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
According to Gudipaty et al "only this Piezo1 antibody reliably worked for Piezo1 immunostaining, as it vanishes in cells mosaically expressing a Piezo1-shRNA-mCherry construct" (PMID: 28199303). In Western Blot, a band is seen ~286 kDa representing PIEZO1 protein by the WB-validated antibody. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Read 1 Review rated 3 using NBP1-78446 in the following applications:
Guinea Pig reactivity reported in scientific literature (PMID: 30324494).
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS, 30% Glycerol
0.05% Sodium Azide
Immunogen affinity purified
Alternate Names for PIEZO1 Antibody
membrane protein induced by beta-amyloid treatment
piezo-type mechanosensitive ion channel component 1
Piezo1 (Mib, FAM38A) is a mechanosensitive ion channel which responds to mechanically activated currents in numerous cells, including the brain, bladder, colon, kidney, lung, and skin. However, mRNA expression of Piezo1 is weak in the DRG, unlike Piezo2. The expression of Piezo1 is induced in Alzheimer's associated senile plaques (PMID 16854388), as well as integrin activation through R-Ras signaling (PMID 20016066). R-Ras is recruited to the endoplasmic reticulum which increases calcium release, leading to the activation of the protease calpain. Piezo proteins are fairly novel proteins, and whose sequences are not akin to other ion channels or proteins.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for PIEZO1 Antibody (NBP1-78446). (Showing 1 - 1 of 1 FAQs).
I am interested in purchasing the PIEZO 1 and PIEZO 2 antibodies and their blocking peptides from Novus. I have read your data sheets that in WBs the bands seen are approximately 286 kDa and 318 kDa, respectively. I would greatly appreciate if you could show me these WBs on human cells For PIEZO1 and 2 and the effects of the blocking peptides (or point me to publications using your Abs).
We offer a PIEZO1 blocking peptide specific to product NBP1-78446. We unfortunately do not offer a PIEZO2 blocking peptide. Our western blot data for NBP1-78446, NBP1-78624, and NBP1-78538 was performed by an outside collaborator and they have not given permission to us to release their images. That is why we do not have them available on our website. We will fully guarantee all of our products for any listed application or species. We unfortunately do not have any publications for these products either.
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