PIEZO1 Antibody

Images

 
Immunocytochemistry: PIEZO1 Antibody [NBP1-78446] - PIEZO1 antibody (Goat anti-rabbit 568) was tested in MDCK cells. Nuclei and B-actin were counterstained with DAPI (blue) and phalloidin (green). Image from verified ...read more
Immunohistochemistry: PIEZO1 Antibody [NBP1-78446] - Analysis of PIEZ01 in mouse epidermis using DAB with hematoxylin counterstain.
Immunocytochemistry/ Immunofluorescence: PIEZO1 Antibody [NBP1-78446] - PIEZO1 antibody was tested in A431 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Product Details

Summary
Reactivity Hu, Mu, GPSpecies Glossary
Applications ICC/IF, IHC, IHC-P, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.12 mg/ml

PIEZO1 Antibody Summary

Immunogen
A synthetic peptide made to an internal portion of the human PIEZO1 protein (between residues 950-1000) [UniProt Q92508]
Localization
Endoplasmic reticulum membrane; Multi-pass membrane protein. Endoplasmic reticulum-Golgi intermediate compartment membrane. Cell membrane; Multi-pass membrane protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PIEZO1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:25
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400
  • Immunocytochemistry
Application Notes
According to Gudipaty et al "only this Piezo1 antibody reliably worked for Piezo1 immunostaining, as it vanishes in cells mosaically expressing a Piezo1-shRNA-mCherry construct" (PMID: 28199303). In Western Blot, a band is seen ~286 kDa representing PIEZO1 protein by the WB-validated antibody. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Control Peptide
Reviewed Applications
Read 1 Review rated 3
using
NBP1-78446 in the following applications:

Publications
Read Publications using
NBP1-78446 in the following applications:

Reactivity Notes

Guinea Pig reactivity reported in scientific literature (PMID: 30324494).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1.12 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PIEZO1 Antibody

  • FAM38A
  • member A
  • membrane protein induced by beta-amyloid treatment
  • Mib
  • piezo-type mechanosensitive ion channel component 1

Background

Piezo1 (Mib, FAM38A) is a mechanosensitive ion channel which responds to mechanically activated currents in numerous cells, including the brain, bladder, colon, kidney, lung, and skin. However, mRNA expression of Piezo1 is weak in the DRG, unlike Piezo2. The expression of Piezo1 is induced in Alzheimer's associated senile plaques (PMID 16854388), as well as integrin activation through R-Ras signaling (PMID 20016066). R-Ras is recruited to the endoplasmic reticulum which increases calcium release, leading to the activation of the protease calpain. Piezo proteins are fairly novel proteins, and whose sequences are not akin to other ion channels or proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PIEZO1 Antibody (NBP1-78446)(4)

Review for PIEZO1 Antibody (NBP1-78446) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-78446:
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ICC
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry PIEZO1 NBP1-78446
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reviewed by:
Swapna A. Gudipaty
ICC Human 06/26/2017
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Summary

ApplicationImmunocytochemistry
Sample TestedMDCK canine kidney epithelial cell line,16 Human bronchial epithelium
SpeciesHuman
LotA-1

Product General Protocols

View specific protocols for PIEZO1 Antibody (NBP1-78446): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for PIEZO1 Antibody (NBP1-78446). (Showing 1 - 1 of 1 FAQs).

  1. I am interested in purchasing the PIEZO 1 and PIEZO 2 antibodies and their blocking peptides from Novus. I have read your data sheets that in WBs the bands seen are approximately 286 kDa and 318 kDa, respectively. I would greatly appreciate if you could show me these WBs on human cells For PIEZO1 and 2 and the effects of the blocking peptides (or point me to publications using your Abs).
    • We offer a PIEZO1 blocking peptide specific to product NBP1-78446. We unfortunately do not offer a PIEZO2 blocking peptide. Our western blot data for NBP1-78446, NBP1-78624, and NBP1-78538 was performed by an outside collaborator and they have not given permission to us to release their images. That is why we do not have them available on our website. We will fully guarantee all of our products for any listed application or species. We unfortunately do not have any publications for these products either.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-78446AF350
Alexa Fluor 405 NBP1-78446AF405
Alexa Fluor 488 NBP1-78446AF488
Alexa Fluor 532 NBP1-78446AF532
Alexa Fluor 594 NBP1-78446AF594
Alexa Fluor 647 NBP1-78446AF647
Alexa Fluor 700 NBP1-78446AF700
Alexa Fluor 750 NBP1-78446AF750
Biotin NBP1-78446B
DyLight 350 NBP1-78446UV
DyLight 405 NBP1-78446V
DyLight 488 NBP1-78446G
DyLight 550 NBP1-78446R
DyLight 594 NBP1-78446DL594
DyLight 650 NBP1-78446C
DyLight 680 NBP1-78446FR
DyLight 755 NBP1-78446IR
FITC NBP1-78446F
HRP NBP1-78446H
Janelia Fluor 549 NBP1-78446JF549
Janelia Fluor 646 NBP1-78446JF646

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Bioinformatics Tool for PIEZO1 Antibody (NBP1-78446)

Discover related pathways, diseases and genes to PIEZO1 Antibody (NBP1-78446). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on PIEZO1.

Touch Infographic: From Touch Receptors to the Brain
The body contains thousands of receptors and nerves which allow us to experience the sense of touch, also referred to as tactile perception. The somatosensory system allows organisms to perceive and decode a wide range of tactile stimuli to allow for...  Read full blog post.

PIEZO1: A Mechanosensitive Ion Channel Protein
PIEZO1, and its close homologue PIEZO2, are mechanosensitive ion channel proteins. Mechanosensitive ion channels couple protein conformation to the mechanics of the surrounding membrane, and switch between a closed state and an open state in response...  Read full blog post.

Recombinant Monoclonal Antibodies

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Recent Reviews

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Swapna A. Gudipaty
06/26/2017
Application: ICC
Species: Human

Bioinformatics

Gene Symbol PIEZO1
Entrez
Uniprot