PIEZO1 Antibody [CoraFluor™ 1]

• Reactivity: Hu, Mu, Gp
• Applications: WB, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: CoraFluor 1

PIEZO1 Antibody [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Immunogen: A synthetic peptide made to an internal portion of the human PIEZO1 protein (between residues 950-1000) [UniProt Q92508]
• Localization: Endoplasmic reticulum membrane; Multi-pass membrane protein. Endoplasmic reticulum-Golgi intermediate compartment membrane. Cell membrane; Multi-pass membrane protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PIEZO1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Guinea Pig reactivity reported in scientific literature (PMID: 30324494).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for PIEZO1 Antibody [CoraFluor™ 1]

• FAM38A
• KIAA0233
• member A
• membrane protein induced by beta-amyloid treatment
• Mib
• PIEZO1
• piezo-type mechanosensitive ion channel component 1
• Protein FAM38A

Background

PIEZO1 (piezo-type mechanosensitive ion channel component 1) plays a crucial role in epithelial cell adhesion. PIEZO1 belongs to the PIEZO family of transmembrane proteins. Mutations in Piezo1 have been tied to Xerocytosis. PIEZO1 has been studied in relation to neuronitis, Alzheimer's disease and Parkinson's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PIEZO1