PIBF1 Antibody - BSA Free

Western Blot: PIBF1 Antibody [NB100-58834] - Detection of Human PIBF1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PIBF1 antibody NB100-58834 used for IP at 3 mcg/mg lysate. PIBF1 was also immunoprecipitated by rabbit anti-PIBF1 antibody NB100-58835, which recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 10 seconds.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

PIBF1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PIBF1 Antibody - BSA Free (NB100-58834) is a polyclonal antibody validated for use in WB, IP and ChIP. Anti-PIBF1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 685 and 735 of human progesterone-induced-blocking factor 1 using the numbering given in entry NP_006337.2 (GeneID 10464).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PIBF1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
• Control:

  PIBF1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PIBF1 Antibody - BSA Free

• C13orf24
• chromosome 13 open reading frame 24
• KIAA1008
• PIBF
• PIBF1
• progesterone immunomodulatory binding factor 1
• progesterone-induced blocking factor 1
• progesterone-induced-blocking factor 1
• RP11-505F3.1

Background

PIBF (progesterone-induced blocking factor 1) is synthesized during pregnancy in response to progesterone by progesterone receptor-positive T lymphocytes (mostly gamma-delta T cells). In the presence of PIBF, natural killer (NK) cells inhibit the release of perforin from storage granules and, therefore, fail to lyse target cells. In humans, the amount of cells that express PIBF is significantly higher in healthy pregnant women than in women at risk for premature pregnancy termination. PIBF has a predicted 89 kDa molecular mass; the full-length PIBF is associated with the nucleus, whereas secretion of shorter forms, such a 34 kDa protein is induced by activation of the cell. Research suggests that PIBF functions as a transcription factor in its full-length form, while smaller forms may act as cytokines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PIBF1 Antibody (NB100-58834)(1)

Publication using NB100-58834

• Li X, Ren C, Huang A et al. PIBF1 regulates multiple gene expression via impeding long-range chromatin interaction to drive the malignant transformation of HPV16 integration epithelial cells Journal of advanced research 2023-05-12 [PMID: 37182685] (ChIP, IP, WB)

Bioinformatics

• Gene Symbol: PIBF1
• Entrez:
  • 10464(Human)
• Uniprot:
  • NP_006337.2(Human)