PIBF1 Overexpression Lysate

Images

 
Western Blot: PIBF1 Overexpression Lysate (Adult Normal) [NBL1-14389] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PIBF1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA

Order Details

PIBF1 Overexpression Lysate Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC208744 and based on accession number NM_006346. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens progesterone immunomodulatory binding factor 1 (PIBF1), mRNA.
Gene
PIBF1

Applications/Dilutions

Dilutions
  • ELISA 2 ug/ml
Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug). ELISA reported in verified customer review.
Theoretical MW
89.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBL1-14389 in the following application:

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for PIBF1 Overexpression Lysate

  • C13orf24
  • chromosome 13 open reading frame 24
  • KIAA1008
  • PIBF
  • PIBF1
  • progesterone immunomodulatory binding factor 1
  • progesterone-induced blocking factor 1
  • progesterone-induced-blocking factor 1
  • RP11-505F3.1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for PIBF1 Lysate (NBL1-14389) (0)

There are no publications for PIBF1 Lysate (NBL1-14389).
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Review for PIBF1 Lysate (NBL1-14389) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBL1-14389:
Filter by Applications
ELISA
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
ELISA Human 08/26/2010
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Summary

ApplicationELISA
Sample Testedhuman blood
SpeciesHuman
Lot62110
CommentsI used it as standard in ELISA assay. It's a sandwich ELISA (pAb, serum, pAb, sAb, substrat). Since I am building this ELISA assay and that I'm not finished, I don't have official results. However, my primary antibody recognize the molecule wich is what I expected.

Primary Anitbody

Dilution RatioPrimary Ab Incubation Dilution Buffer: PBS 1X, Primary Ab Dilution Ratio: 2 ug/mL, Incubation Time:overnight, Incubation Temp:4C

Secondary Antibody

Secondary DescriptionSecondary Ab: goat anti rabbit, Secondary Ab Dilution: 1:3000
Secondary Manufacturer Cat#1721019 BioRad

Details

Detection NotesPositive Control: PIBF lysate, Negative Control: PBS-BSA 0.5%, Wavelength: 450 nM
ELISA Plate DetailsELISA Detection Method: TMB, ELISA Sample Concentration: pure or 1:5

Comments

CommentsI used it as standard in ELISA assay. It's a sandwich ELISA (pAb, serum, pAb, sAb, substrat). Since I am building this ELISA assay and that I'm not finished, I don't have official results. However, my primary antibody recognize the molecule wich is what I expected.

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Video Protocols

WB Video Protocol

FAQs for PIBF1 Lysate (NBL1-14389) (0)

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Other Available Formats

NBL1-14389

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Recent Reviews

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Anonymous
08/26/2010
Application: ELISA
Species: Human

Bioinformatics

Gene Symbol PIBF1