PIASy Antibody

Images

 
Western Blot: PIASy Antibody [NBP1-31215] - A. 30 ug 293T whole cell lysate/extract B. 30 ug whole cell lysate/extract of human PIAS4-transfected 293T cells 10% SDS-PAGE PIASy antibody dilution: 1:5000 The ...read more
Immunocytochemistry/ Immunofluorescence: PIASy Antibody [NBP1-31215] - Paraformaldehyde-fixed HeLa, using PIASy antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody ...read more
Immunohistochemistry: PIASy Antibody [NBP1-31215] - PIASy antibody detects PIASy protein at nucleus in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. PIASy antibody diluted at 1:500.
Immunohistochemistry-Paraffin: PIASy Antibody [NBP1-31215] - Paraffin-embedded DLD1 Xenograft, using antibody at 1:100 dilution.

Product Details

Summary
Reactivity Hu, Mu, Bv, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

PIASy Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PIASy. The exact sequence is proprietary.
Localization
Nucleus
Predicted Species
Bovine (100%), Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PIAS4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:10000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP1-31215 in the following applications:

Reactivity Notes

Chimpanzee (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PIASy Antibody

  • E3 SUMO-protein ligase PIAS4
  • FLJ12419
  • MGC35296
  • Piasg
  • PIAS-gamma
  • PIASY
  • Protein inhibitor of activated STAT protein 4
  • Protein inhibitor of activated STAT protein gamma
  • protein inhibitor of activated STAT protein PIASy
  • protein inhibitor of activated STAT, 4
  • zinc finger, MIZ-type containing 6
  • ZMIZ6

Background

The IL-6-type family of cytokines, which includes IL-6 as well as a number of similar cytokines and growth factors, plays a significant role in regulating gene activation, proliferation and differentiation. Transcription factors of the Stat family are known to be involved in this signal transduction pathway, undergoing phosphorylation, dimerization and translocation to the nucleus upon activation. PIAS 1, for protein inhibitor of activated Stat1 (also designated Gu/RNA helicase II binding protein), binds specifically to Stat1, blocking Stat1 DNA-binding activity and inhibiting Stat1-mediated gene activation. PIAS 1 also binds to the Gu/RNA helicase II enzyme, leading to the proteolytic cleavage of Gu/RH-II. PIAS 3 similarly binds specifically to Stat3, blocking Stat3 DNA-binding activity and inhibiting Stat3-mediated gene activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PIASy Antibody (NBP1-31215)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-P.


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IHC-P
(1)
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Filter By Species
Human
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PIAS4