Western Blot: PIASy Antibody [NBP1-31215] - Sample(30 ug whole cell lysate) A:HeLa S3 B:Hep G2 7.5% SDS PAGE, antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: PIASy Antibody [NBP1-31215] - Paraformaldehyde-fixed HeLa, using PIASy antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody ...read more
Immunohistochemistry: PIASy Antibody [NBP1-31215] - PIASy antibody detects PIASy protein at nucleus in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. PIASy antibody diluted at 1:500.
Immunohistochemistry-Paraffin: PIASy Antibody [NBP1-31215] - Paraffin-embedded DLD1 Xenograft, using antibody at 1:100 dilution.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publication using NBP1-31215 in the following applications:
The IL-6-type family of cytokines, which includes IL-6 as well as a number of similar cytokines and growth factors, plays a significant role in regulating gene activation, proliferation and differentiation. Transcription factors of the Stat family are known to be involved in this signal transduction pathway, undergoing phosphorylation, dimerization and translocation to the nucleus upon activation. PIAS 1, for protein inhibitor of activated Stat1 (also designated Gu/RNA helicase II binding protein), binds specifically to Stat1, blocking Stat1 DNA-binding activity and inhibiting Stat1-mediated gene activation. PIAS 1 also binds to the Gu/RNA helicase II enzyme, leading to the proteolytic cleavage of Gu/RH-II. PIAS 3 similarly binds specifically to Stat3, blocking Stat3 DNA-binding activity and inhibiting Stat3-mediated gene activation.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.