PIAS3 Antibody - BSA Free

Images

 
Western Blot: PIAS3 Antibody [NBP1-77160] - K562 cell lysate with PIAS3 antibody at 1 ug/mL.
Immunohistochemistry-Paraffin: PIAS3 Antibody [NBP1-77160] - Human breast carcinoma tissue with PIAS3 antibody at 5 ug/mL.
Western Blot: PIAS3 Antibody [NBP1-77160] - Western for PIAS3 in THP1 macrophage cells, Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

PIAS3 Antibody - BSA Free Summary

Immunogen
Antibody was raised against a 12 amino acid synthetic peptide near the carboxy terminus of human PIAS3. The immunogen is located within amino acids 410 - 460 of PIAS3. Amino Acid Squence: SPVQGGDPSENK
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PIAS3
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1 ug/ml
Control Peptide
PIAS3 Peptide (NBP1-77160PEP)
Reviewed Applications
Read 1 Review rated 4
using
NBP1-77160 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for PIAS3 Antibody - BSA Free

  • MIZ-type containing 5
  • PIAS3
  • Protein inhibitor of activated STAT protein 3
  • protein inhibitor of activated STAT, 3
  • ZMIZ5

Background

The PIAS (protein inhibitor of activated STAT) proteins play a crucial role as transcriptional coregulators in various cellular pathways, including the STAT, p53 and the steroid hormone signaling pathway. The PIAS protein family includes at least five evolutionarily conserved genes, including PIAS3. The major function of the PIAS proteins is the control of gene transcription and can also act as small ubiquitin-like-modifier (SUMO) E3 ligases. PIAS3 binds specifically to STAT3 following the stimulation of STAT3. Increased expression of PIAS3 has been observed in several human cancers, including lung, breast, and brain tumors, but not in anaplastic lymphoma kinase-positive T/null-cell lymphomas, indicating that PIAS3 plays multiple roles in different tissue and cell types.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PIAS3 Antibody (NBP1-77160) (0)

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Review for PIAS3 Antibody (NBP1-77160) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-77160:
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Images Ratings Applications Species Date Details
Western Blot PIAS3 NBP1-77160
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4
reviewed by:
James Crocket
WB Human 09/26/2014
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Summary

ApplicationWestern Blot
Sample TestedSample tested: THP1 cells
SpeciesHuman

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Secondary Antibodies

 

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Recent Reviews

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4
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2
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1
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James Crocket
09/26/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol PIAS3
Uniprot