PIAS1 Antibody - BSA Free

Images

 
Western Blot: PIAS1 Antibody [NBP1-77158] - human kidney tissue lysate with PIAS1 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry: PIAS1 Antibody [NBP1-77158] - Rat kidney tissue at 20 ug/mL.
Immunohistochemistry-Paraffin: PIAS1 Antibody [NBP1-77158] - Rat kidney tissue with PIAS1 antibody at 5 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

PIAS1 Antibody - BSA Free Summary

Immunogen
Antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human PIAS1. The immunogen is located within the last 50 amino acids of PIAS1. Amino Acid Squence: SNSLRESHSHTVTNR
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PIAS1
Purity
Peptide affinity purified
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1 ug/ml
Control Peptide
PIAS1 Peptide (NBP1-77158PEP)
Reviewed Applications
Read 1 Review rated 3
using
NBP1-77158 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for PIAS1 Antibody - BSA Free

  • AR interacting protein
  • DDXBP1MGC141878
  • DEAD/H (Asp-Glu-Ala-Asp/His) box binding protein 1
  • DEAD/H box-binding protein 1
  • E3 SUMO-protein ligase PIAS1
  • GBPRNA helicase II-binding protein
  • GU/RH-II
  • Gu-binding protein
  • MGC141879
  • Protein inhibitor of activated STAT protein 1
  • protein inhibitor of activated STAT, 1
  • protein inhibitor of activated STAT-1
  • zinc finger, MIZ-type containing 3
  • ZMIZ3

Background

The PIAS proteins (protein inhibitor of activated STAT) play a crucial role as transcriptional coregulators in various cellular pathways, including the STAT, p53 and the steroid hormone signaling pathway. The PIAS protein family includes at least five evolutionarily conserved genes, including PIAS1. The major function of the PIAS proteins is the control of gene transcription and can also act as small ubiquitin-like-modifier (SUMO) E3 ligases. PIAS1 binds specifically to STAT1, inhibiting STAT1-mediated gene activation and also binds to the Gu/RNA helicase II enzyme, leading to the proteolytic cleavage of Gu/RH-II. PIAS1 is a potent co-activator for CP2c-mediated alpha-globin expression in erythroid cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PIAS1 Antibody (NBP1-77158) (0)

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Review for PIAS1 Antibody (NBP1-77158) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-77158:
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Images Ratings Applications Species Date Details
Western Blot PIAS1 NBP1-77158
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3
reviewed by:
James Crocket
WB Human 09/25/2014
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Summary

ApplicationWestern Blot
Sample TestedTHP1 cells
SpeciesHuman

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PIAS1 Antibody (NBP1-77158) (0)

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Secondary Antibodies

 

Isotype Controls

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James Crocket
09/25/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol PIAS1
Uniprot