PIAS1 Antibody

Images

 
Western Blot: PIAS1 Antibody [NBP1-77158] - human kidney tissue lysate with PIAS1 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry-Paraffin: PIAS1 Antibody [NBP1-77158] - Rat kidney tissue with PIAS1 antibody at 5 ug/mL.
Immunofluorescence: PIAS1 Antibody [NBP1-77158] - Rat kidney tissue at 20 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

PIAS1 Antibody Summary

Immunogen
Antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human PIAS1. The immunogen is located within the last 50 amino acids of PIAS1.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PIAS1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunofluorescence 20 ug/ml
Control Peptide
PIAS1 Peptide (NBP1-77158PEP)
Reviewed Applications
Read 1 Review rated 3
using
NBP1-77158 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PIAS1 Antibody

  • AR interacting protein
  • DDXBP1MGC141878
  • DEAD/H (Asp-Glu-Ala-Asp/His) box binding protein 1
  • DEAD/H box-binding protein 1
  • E3 SUMO-protein ligase PIAS1
  • GBPRNA helicase II-binding protein
  • GU/RH-II
  • Gu-binding protein
  • MGC141879
  • Protein inhibitor of activated STAT protein 1
  • protein inhibitor of activated STAT, 1
  • protein inhibitor of activated STAT-1
  • zinc finger, MIZ-type containing 3
  • ZMIZ3

Background

The PIAS proteins (protein inhibitor of activated STAT) play a crucial role as transcriptional coregulators in various cellular pathways, including the STAT, p53 and the steroid hormone signaling pathway. The PIAS protein family includes at least five evolutionarily conserved genes, including PIAS1. The major function of the PIAS proteins is the control of gene transcription and can also act as small ubiquitin-like-modifier (SUMO) E3 ligases. PIAS1 binds specifically to STAT1, inhibiting STAT1-mediated gene activation and also binds to the Gu/RNA helicase II enzyme, leading to the proteolytic cleavage of Gu/RH-II. PIAS1 is a potent co-activator for CP2c-mediated alpha-globin expression in erythroid cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PIAS1 Antibody (NBP1-77158) (0)

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Review for PIAS1 Antibody (NBP1-77158) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-77158:
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Images Ratings Applications Species Date Details
Western Blot PIAS1 NBP1-77158
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reviewed by:
Anonymous
WB Human 09/25/2014
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Summary

ApplicationWestern Blot
Sample TestedTHP1 cells
SpeciesHuman
CommentsThe signal for PIAS1 is very less due to the less abundance of the protein in THP1 cells

Blocking

Blocking DetailsBSA (5%) in PBST

Primary Anitbody

Dilution Ratio1:1K in PBST buffer

Secondary Antibody

Secondary DescriptionGoat anti-Rabbit IgG Antibody (HRP)-Novus
Secondary Manufacturer Cat#Cat No NB7160
Secondary ConcentrationDiluted 20,000 times

Details

Detection NotesDetected using PromoKine ECL Chemiluminescence Detection Kit with a 1 min exposure on X-ray film

Comments

CommentsThe signal for PIAS1 is very less due to the less abundance of the protein in THP1 cells

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PIAS1 Antibody (NBP1-77158) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for PIAS1 Antibody (NBP1-77158)

Discover related pathways, diseases and genes to PIAS1 Antibody (NBP1-77158). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for PIAS1 Antibody (NBP1-77158)

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Research Areas for PIAS1 Antibody (NBP1-77158)

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Blogs on PIAS1

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Recent Reviews

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1
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Anonymous
09/25/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol PIAS1
Uniprot