Phosphoserine phosphatase Antibody (001) - Azide and BSA Free

Western Blot: Phosphoserine phosphatase Antibody (001) [NBP2-90222] - Anti-PSPH rabbit monoclonal antibody at 1:500 dilution. Lane A: MCF7 Whole Cell Lysate. Lane B: K562 Whole Cell Lysate. Lane C: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:25 kDa. Observed band size:27 kDa

Immunoprecipitation: Phosphoserine phosphatase Antibody (001) [NBP2-90222] - PSPH was immunoprecipitated using: Lane A:0.5 mg K562 Whole Cell Lysate. Lane B:0.5 mg HepG2 Whole Cell Lysate. 0.5 uL anti-PSPH rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-PSPH rabbit monoclonal antibody,at 1:1000 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions.Predicted band size: 25 kDa. Observed band size: 25 kDa.

Immunocytochemistry/Immunofluorescence: Phosphoserine phosphatase Antibody (001) [NBP2-90222] - Immunofluorescence staining of Human PSPH in A549 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-90222 antibody (dilution ratio 1:60) at 4? overnight. Then cells were stained with the Alexa Fluor(R) 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue).

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IP
• Clone: 001
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Phosphoserine phosphatase Antibody (001) - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was obtained from a rabbit immunized with purified, recombinant Human Phosphoserine phosphatase (Uniprot#: P78330; Met1-Glu225).
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PSPH
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:25000-1:50000
  • Immunocytochemistry/ Immunofluorescence 1:20-1:100
  • Immunoprecipitation 0.2-1 uL/mg of lysate
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for Phosphoserine phosphatase Antibody (001) - Azide and BSA Free

• L-3-phosphoserine phosphatase
• O-phosphoserine phosphohydrolase
• phosphoserine phosphatase
• PSPase
• PSPEC 3.1.3.3

Background

Phosphoserine phosphatase is encoded by this gene belongs to a subfamily of the phosphotransferases. This encoded enzyme is responsible for the third and last step in L-serine formation. It catalyzes magnesium-dependent hydrolysis of L-phosphoserine and is also involved in an exchange reaction between L-serine and L-phosphoserine. Deficiency of this protein is thought to be linked to Williams syndrome. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PSPH