Phosphoribosyl Pyrophosphate Amidotransferase Overexpression Lysate (Native)

Images

 
Western Blot: Phosphoribosyl Pyrophosphate Amidotransferase Overexpression Lysate (Adult Normal) [NBL1-14638] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Phosphoribosyl Pyrophosphate Amidotransferase Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC201144 and based on accession number NM_002703. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens phosphoribosyl pyrophosphate amidotransferase (PPAT), mRNA.
Gene
PPAT

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
57.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Phosphoribosyl Pyrophosphate Amidotransferase

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Phosphoribosyl Pyrophosphate Amidotransferase Overexpression Lysate (Native)

  • ATASE
  • EC 2.4.2.14
  • glutamine phosphoribosylpyrophosphatate amidotransferase
  • Glutamine phosphoribosylpyrophosphate amidotransferase
  • glutamine PRPP amidotransferase
  • GPATamidophosphoribosyltransferase
  • phosphoribosyl pyrophosphate amidotransferase
  • PRAT

Background

The protein encoded by this gene is a member of the purine/pyrimidine phosphoribosyltransferase family. This protein is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosynthesis. This gene and PAICS/AIRC, a bifunctional enzyme catalyzing steps six and seven in the purine nucleotide biosynthesis pathway, are located in close proximity on chromosome 4. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB110-41404
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC
NB400-114
Species: Hu, Mu, Rt, Po, Ch, Ha, Pl, Pm
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP1-81838
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
NBP1-89519
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB120-13550
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-90235
Species: Hu, Mu, Rt, ChHa
Applications: WB, Simple Western, IHC, IHC-P
H00008443-B01P
Species: Hu
Applications: WB, ICC/IF
NBP1-33527
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-87404
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP1-91705
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-83107
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-29940
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC-P
NB600-935
Species: Bv
Applications: WB, ELISA, IP
NBP2-22106
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NB110-41487
Species: Hu, Mu, Rt, Bv, Ze
Applications: WB, ICC/IF
NB600-582
Species: Hu, Mu, Rt, Ca, Ch, SyHa, Ha
Applications: WB, Simple Western
NBL1-14638
Species: Hu
Applications: WB

Publications for Phosphoribosyl Pyrophosphate Amidotransferase Lysate (NBL1-14638) (0)

There are no publications for Phosphoribosyl Pyrophosphate Amidotransferase Lysate (NBL1-14638).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Phosphoribosyl Pyrophosphate Amidotransferase Lysate (NBL1-14638) (0)

There are no reviews for Phosphoribosyl Pyrophosphate Amidotransferase Lysate (NBL1-14638). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Phosphoribosyl Pyrophosphate Amidotransferase Lysate (NBL1-14638) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Phosphoribosyl Pyrophosphate Amidotransferase Products

Bioinformatics Tool for Phosphoribosyl Pyrophosphate Amidotransferase Lysate (NBL1-14638)

Discover related pathways, diseases and genes to Phosphoribosyl Pyrophosphate Amidotransferase Lysate (NBL1-14638). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Phosphoribosyl Pyrophosphate Amidotransferase Lysate (NBL1-14638)

Discover more about diseases related to Phosphoribosyl Pyrophosphate Amidotransferase Lysate (NBL1-14638).
 

Pathways for Phosphoribosyl Pyrophosphate Amidotransferase Lysate (NBL1-14638)

View related products by pathway.

PTMs for Phosphoribosyl Pyrophosphate Amidotransferase Lysate (NBL1-14638)

Learn more about PTMs related to Phosphoribosyl Pyrophosphate Amidotransferase Lysate (NBL1-14638).
 

Research Areas for Phosphoribosyl Pyrophosphate Amidotransferase Lysate (NBL1-14638)

Find related products by research area.

Blogs on Phosphoribosyl Pyrophosphate Amidotransferase

There are no specific blogs for Phosphoribosyl Pyrophosphate Amidotransferase, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Phosphoribosyl Pyrophosphate Amidotransferase Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol PPAT