Phospholipid Scramblase 1/PLSCR1 Antibody (875327)

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Phospholipid Scramblase 1/PLSCR1 Monoclonal Antibody (Catalog # MAB8137) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Phospholipid Scramblase 1/PLSCR1 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Phospholipid Scramblase 1/PLSCR1 was detected in immersion fixed HT-29 human colon adenocarcinoma cell line using Mouse Anti-Human Phospholipid Scramblase 1/PLSCR1 Monoclonal Antibody (Catalog # MAB8137) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 875327
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Phospholipid Scramblase 1/PLSCR1 Antibody (875327) Summary

• Immunogen: E. coli-derived recombinant human Phospholipid Scramblase 1/PLSCR1
  Met1-Pro84
  Accession # O15162
• Specificity: Detects human Phospholipid Scramblase 1/PLSCR1 in ELISA.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PLSCR1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Phospholipid Scramblase 1/PLSCR1 Antibody (875327)

• Erythrocyte phospholipid scramblase
• MMTRA1B
• MMTRA1BCa(2+)-dependent phospholipid scramblase 1
• Phospholipid Scramblase 1
• PL scramblase 1
• PLSCR1

Background

PLSCR1 (phospholipid scramblase 1) is an approximately 37 kDa, type II transmembrane protein that is a member of the Ca2+-dependent phospholipid scramblase family that regulates phospholipid asymmetry of membranes. It is most highly expressed on erythrocytes and platelets, and plays roles in exocytosis and apoptosis. PLSCR1 also has nuclear activity, for example, as an activator of genes in response to interferons. The 318 amino acid (aa) human PLSCR1 contains a 288 aa cytoplasmic domain with three SH3-binding and two WW-binding adaptor domains, three phosphorylation sites, four potential palmitoylation sites (regulating localization to the nucleus or plasma membrane) and a nuclear localization signal, followed by a transmembrane domain and a short extracellular domain. The N-terminus of human PLSCR1 (aa 1-84) shares <60% aa sequence identity with mouse and rat PLSCR1. Potential isoforms have a downstream start site without (311 aa) or with (186 aa) divergence after aa 192, or an upstream start site with divergence of 1 or 11 aa after aa 192 (205, 215 aa).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Phospholipid Scramblase 1/PLSCR1 Antibody (MAB8137)(3)

Publications using MAB8137

• Yang, AX;Ramos-Rodriguez, L;Sorkhdini, P;Yang, D;Norbrun, C;Majid, S;Zhang, Y;Holtzman, MJ;Boyd, DF;Zhou, Y; Phospholipid Scramblase 1 (PLSCR1) Regulates Interferon-Lambda Receptor 1 (IFN-?R1) and IFN-? Signaling in Influenza A Virus (IAV) Infection bioRxiv : the preprint server for biology 2024-11-21 [PMID: 39605457] (Immunocytochemistry, Human)
• Li D, Sun H, Bai G et al. a-1,3-Fucosyltransferase-VII siRNA inhibits the expression of SLex and hepatocarcinoma cell proliferation. Int. J. Mol. Med. 11/1/2018 [PMID: 30226570]
• Park Y, Kang J, Lee D, Kim M, Bak Y, Yang Y, Lee H, Hong J, Yoon D Interleukin-32alpha modulates promyelocytic leukemia zinc finger gene activity by inhibiting protein kinase Cvarepsilon-dependent sumoylation. Int J Biochem Cell Biol, 2014-08-29;55(0):136-43. 2014-08-29 [PMID: 25178676] (Western Blot, Human)

Bioinformatics

• Gene Symbol: PLSCR1
• Uniprot:
  • O15162()