PGLS Antibody

Western Blot: PGLS Antibody [NBP2-19785] - Sample (30 ug of whole cell lysate) A: SK-N-SH 12% SDS PAGE gel, diluted at 1:5000.

Immunocytochemistry/Immunofluorescence: PGLS Antibody [NBP2-19785] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PGLS protein stained by PGLS antibody diluted at 1:500. Blue: Hoechst 33342 staining.Scale bar = 10 um.

Immunohistochemistry-Paraffin: PGLS Antibody [NBP2-19785] - : Paraffin-embedded mouse prostate. PGLS antibody dilution: 1:500.

Immunohistochemistry-Paraffin: PGLS Antibody [NBP2-19785] - Immunohistochemical analysis of paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.

• Reactivity: Hu, Mu, Bv
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

PGLS Antibody Summary

• Description: Novus Biologicals Rabbit PGLS Antibody (NBP2-19785) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-PGLS Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human PGLS. The exact sequence is proprietary.
• Predicted Species: Bovine (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PGLS
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:1000-1:10000
• Theoretical MW: 28 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (88%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for PGLS Antibody

• 6PGLEC 3.1.1.31
• 6-phosphogluconolactonase

Background

Hydrolysis of 6-phosphogluconolactone to 6-phosphogluconate.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for PGLS Antibody (NBP2-19785)(1)

Publication using NBP2-19785

• Pushpak Bhattacharjee, Die Wang, Dovile Anderson, Joshua N Buckler, Eveline de Geus, Feng Yan, Galina Polekhina, Ralf Schittenhelm, Darren J Creek, Lawrence D Harris, Anthony J Sadler The immune response to RNA suppresses nucleic acid synthesis by limiting ribose 5-phosphate The EMBO Journal 2024-05-22 [PMID: 38778156]

Bioinformatics

• Gene Symbol: PGLS
• Uniprot:
  • O95336(Human)