Sec8 Antibody (14G1)

Images

 
Western Blot: Sec8 Antibody (14G1) [NBP1-97492] - Lane 1: MWM, Lane 2: Brain (rat), (tissue extract), Lane 3: Brain (mouse), (tissue extract), Lane 4: MDBK Cell Lysate.
Immunohistochemistry-Paraffin: Sec8 Antibody (14G1) [NBP1-97492] - Analysis of human brain, cerebellum tissue stained with rSec8, mAb (14G1) at 5ug/ml.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, Ch, Fi, GP, Ha, Pm, Rb, ShSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clone
14G1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Sec8 Antibody (14G1) Summary

Immunogen
Recombinant rat rSec8.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
EXOC4
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 12.5 ug/ml
Application Notes
This antibody is useful in Immunocytochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation and detects a band of ~110kDa by Western blot.
Publications
Read Publications using
NBP1-97492 in the following applications:

Reactivity Notes

Human, Mouse, Rat, Bovine, Canine, Chicken, Fish, Guinea Pig, Hamster, Primate, Pig, Rabbit and Sheep. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 50% Glycerol
Preservative
0.09% Sodium Azide
Purity
Protein G purified

Alternate Names for Sec8 Antibody (14G1)

  • exocyst complex component 4
  • KIAA1699SEC8L1
  • MGC27170
  • REC8
  • SEC8Exocyst complex component Sec8
  • SEC8-like 1 (S. cerevisiae)
  • SEC8-like 1
  • Sec8p

Background

rSec6 and rSec8 are rat homologs of the yeast Sec6 and Sec8 proteins that are essential for protein transport between the Golgi apparatus and the plasma membrane. In yeast, Sec8 is part of a 19.5S complex that includes Sec6 and Sec15, that also associate with the ras-like GTP binding protein Sec4. An analogous 17S complex in rat brain was found to contain rSec6 and rSec8, along with eight proteins with a combined molecular weight of 743 kDa. The membrane-associated rSec6/8 complex co-immunoprecipitates with syntaxin, providing evidence that this complex is involved in synaptic vesicle docking and fusion at the presynaptic terminal.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ICC/IF, IHC, IHC-P, IP

Publications for Sec8 Antibody (NBP1-97492)(3)

We have publications tested in 3 confirmed species: Mouse, Rat, Canine.

We have publications tested in 2 applications: ICC/IF, WB.


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ICC/IF
(1)
WB
(3)
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Rat
(1)
Canine
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol EXOC4