PGK1 Antibody (4B3Q3)

Western Blot: PGK1 Antibody (4B3Q3) [NBP3-15453] - Western blot analysis of extracts of various cell lines, using PGK1 Rabbit mAb (NBP3-15453) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 4B3Q3
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

PGK1 Antibody (4B3Q3) Summary

• Description: Novus Biologicals Rabbit PGK1 Antibody (4B3Q3) (NBP3-15453) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PGK1 (P00558). MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAVPSIKFCLDNGAKSVVLMSHLGRPDGVPMPDKYSLEPVAVELKSLLGKDVLFLKDCV
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PGK1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for PGK1 Antibody (4B3Q3)

• Cell migration-inducing gene 10 protein
• EC 2.7.2.3
• MGC142128
• MGC8947
• MIG10
• PGK1
• PGKA
• PGKAMGC117307
• phosphoglycerate kinase 1
• Primer recognition protein 2
• PRP 2
• PRP2

Background

PGK1 is encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. This gene lies on the X-chromosome, while a related pseudogene also has been found on the X-chromosome and another on chromosome 19. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PGK1