PGD2 Synthase/PTGDS Antibody

Western blot shows lysates rat brain and spinal cord tissue. PVDF membrane was probed with 1 µg mL of Goat Anti-Rat PGD2 Synthase PTGDS Antigen Affinity-purified Polyclonal (Catalog # AF5000) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PGD2 Synthase/PTGDS at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

PGD2 Synthase/PTGDS Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant rat PGD2 Synthase/PTGDS
  Gln25-Glu189
  Accession # P22057
• Specificity: Detects rat PGD2 Synthase/PTGDS in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PTGDS
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PGD2 Synthase/PTGDS Antibody

• Beta-trace protein
• Cerebrin-28
• EC 5.3.99.2
• Glutathione-independent PGD synthase
• glutathione-independent PGD synthetase
• lipocalin-type prostaglandin D synthase
• Lipocalin-type prostaglandin-D synthase
• L-PGDS
• PDS
• PGD2 Synthase
• PGD2
• PGDS2
• PGDSLPGDS
• PH2DISO
• prostaglandin D synthase
• prostaglandin D2 synthase (21kD, brain)
• prostaglandin D2 synthase 21kDa (brain)
• Prostaglandin-D2 synthase
• prostaglandin-H2 D-isomerase
• PTGDS

Background

PGD2 Synthase (prostaglandin D2 synthase; also PG-H2 D-isomerase) is a 26 kDa, monomeric member of the lipocalin family of proteins. It is expressed by neurons and astrocytes, retinal pigment epithelium and Sertoli cells. It catalyzes the conversion of PGH₂ to PGD₂, thereby influencing sleep and body temperature. It also transports retinoic acid. The rat PGD2 precursor is 189 amino acids (aa) in length. It contains a signal sequence (aa 1‑24) plus a lipocalin region that serves both as a catalytic site and hydrophobic molecule transporter (aa 40‑187). PGD2 is both intracellular and secreted. When secreted, PGD2 is called beta -Trace. One splice variant is likely intracellular and shows an 11 aa substitution for the first 52 amino acids. Over aa 25‑189, rat PGD2 shares 72% and 88% aa identity with human and mouse PGD2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PTGDS
• Uniprot:
  • P22057()