PGAM2 Antibody (OTI4E9) - Azide and BSA Free

Western Blot: PGAM2 Antibody (OTI4E9) - Azide and BSA Free [NBP2-73358] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PGAM2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PGAM2.

Immunocytochemistry/Immunofluorescence: PGAM2 Antibody (OTI4E9) - Azide and BSA Free [NBP2-73358] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PGAM2.

Immunohistochemistry: PGAM2 Antibody (OTI4E9) - Azide and BSA Free [NBP2-73358] - Staining of paraffin-embedded Human liver tissue using anti-PGAM2 mouse monoclonal antibody.

Flow Cytometry: PGAM2 Antibody (OTI4E9) - Azide and BSA Free [NBP2-73358] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-PGAM2 antibody, and then analyzed by flow cytometry.

Western Blot: PGAM2 Antibody (OTI4E9) - Azide and BSA Free [NBP2-73358] - Analysis of extracts (35ug) from 9 different cell lines by using anti-PGAM2 monoclonal antibody.

Immunocytochemistry/Immunofluorescence: PGAM2 Antibody (OTI4E9) - Azide and BSA Free [NBP2-73358] - Staining of HeLa cells using anti-PGAM2 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt, Pm, Pm
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OTI4E9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

PGAM2 Antibody (OTI4E9) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human PGAM2(NP_000028) produced in HEK293 cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: PGAM2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-2000
• Theoretical MW: 28.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for PGAM2 Antibody (OTI4E9) - Azide and BSA Free

• BPG-dependent PGAM 2
• EC 3.1.3.13
• EC 5.4.2.1
• EC 5.4.2.4
• Muscle-specific phosphoglycerate mutase
• PGAM-MGSD10
• PGAMMMGC88743
• phosphoglycerate mutase 2 (muscle)
• phosphoglycerate mutase 2
• Phosphoglycerate mutase isozyme M

Background

Phosphoglycerate mutase (PGAM) catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB). This gene encodes muscle-specific PGAM subunit. Mutations in this gene cause muscle phosphoglycerate mutase eficiency, also known as glycogen storage disease X. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PGAM2