PFKFB2 Antibody [mFluor Violet 500 SE]

• Reactivity: Hu, Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: mFluor Violet 500 SE

PFKFB2 Antibody [mFluor Violet 500 SE] Summary

• Immunogen: The immunogen this antibody was made to, maps to a region between residue 455 to 505 of human 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 using the numbering given in entry NP_006203.2 (GeneID 5208).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PFKFB2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for PFKFB2 Antibody [mFluor Violet 500 SE]

• 6PF-2-K/Fru-2,6-P2ase 2
• 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2
• 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2
• DKFZp781D2217
• fructose-2,6-bisphosphatase, cardiac isozyme
• MGC138308,6PF-2-K/Fru-2,6-P2ase heart-type isozyme
• MGC138310
• PFK/FBPase 2
• PFK-2/FBPase-2
• PFKFB, cardiac

Background

Phosphofructokinases (PFK) are regulatory glycolytic enzymes that convert fructose 6-phosphate and ATP into fructose 1,6-bisphosphate (through PFK-1), fructose 2,6-bisphosphate (through PFK-2) and ADP. Human PFK-1 is tetrameric and isoenzymes include, PFK-1 muscle (PFKM, PFK-A), PFK-1 liver (PFKL, PFK-B) and PFK-1 platelet (PFKP, PFK-C, PFKF). PFK-1 is inhibited by ATP and citrate (from the tricarboxylic acid cycle). PFK-1 undergoes activation in the presence of elevated AMP. The most potent activator is fructose 2,6-bisphosphate, which is produced by PFK-2 from the same substrate, fructose 6-phosphate. PFK-2 is bifunctional and a key regulator for PFK-1. PFK-2 catalyzes the synthesis of fructose 2,6-bisphosphate and contains fructose 2,6-biphosphatase activity that catalyzes the degradation of fructose 2,6-bisphosphate. PFK-2 is dimeric and isoenzymes include PFK-2 liver (PFKFB1, PFRX), PFK-2 cardiac (PFKFB2), PFK-2 placental (PFKFB3, inducible PFK-2) and PFK-2 testis (PFKFB4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PFKFB2