| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38605-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33584-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-57257-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38640-20ul | |||
![Immunohistochemistry-Paraffin: PFKFB2 Antibody [NBP1-45238] - 2.5ug/ml staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/PFKFB2-Antibody-Immunohistochemistry-Paraffin-NBP1-45238-img0001.jpg "Immunohistochemistry-Paraffin: PFKFB2 Antibody [NBP1-45238] - 2.5ug/ml staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Immunocytochemistry/ Immunofluorescence: PFKFB2 Antibody [NBP3-38605] - Immunofluorescence analysis of L929 cells using PFKFB2 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38605_rabbit-pfkfb2-pab-1012202411421043.jpg "Immunocytochemistry/ Immunofluorescence: PFKFB2 Antibody [NBP3-38605] - Immunofluorescence analysis of L929 cells using PFKFB2 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: PFKFB2 Antibody [NBP3-38605] - Western blot analysis of various lysates using PFKFB2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-38605_rabbit-pfkfb2-pab-1012202411343351.jpg)
![Immunocytochemistry/ Immunofluorescence: PFKFB2 Antibody [NBP3-38605] - Immunofluorescence analysis of C6 cells using PFKFB2 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38605_rabbit-pfkfb2-pab-101220241136032.jpg)
![Western Blot: PFKFB2 Antibody [NBP2-32194] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-PFKFB2 antibody NBP2-32194 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/PFKFB2-Antibody-Western-Blot-NBP2-32194-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: PFKFB2 Antibody [NBP2-32194] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-PFKFB2 antibody NBP2-32194 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: PFKFB2 Antibody [NBP2-32194] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-PFKFB2 antibody NBP2-32194 used for IP at 6 ug per reaction. PFKFB2 was also immunoprecipitated by rabbit anti-PFKFB2 antibody BL15840. For blotting immunoprecipitated PFKFB2, NBP2-32194 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/PFKFB2-Antibody-Immunoprecipitation-NBP2-32194-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: PFKFB2 Antibody [NBP2-33584] - Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/PFKFB2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33584-img0005.jpg "Immunocytochemistry/Immunofluorescence: PFKFB2 Antibody [NBP2-33584] - Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm.")
![Immunohistochemistry: PFKFB2 Antibody [NBP2-33584] - Staining of human cerebral cortex shows strong cytoplasmic and nuclear positivity in astrocytes.](http://images.novusbio.com/fullsize/PFKFB2-Antibody-Immunohistochemistry-NBP2-33584-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PFKFB2 Antibody [NBP2-57257] - Staining of human cell line SiHa shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/PFKFB2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57257-img0001.jpg "Immunocytochemistry/Immunofluorescence: PFKFB2 Antibody [NBP2-57257] - Staining of human cell line SiHa shows localization to nucleoplasm.")
![Western Blot: PFKFB2 Antibody [NBP1-79635] - Mouse Heart Lysate 1ug/ml Gel Concentration 12%](http://images.novusbio.com/fullsize/PFKFB2-Antibody-Western-Blot-NBP1-79635-img0001.jpg "Western Blot: PFKFB2 Antibody [NBP1-79635] - Mouse Heart Lysate 1ug/ml Gel Concentration 12%")
![Western Blot: PFKFB2 Antibody [NBP1-79636] - Titration: 1.0 ug/ml Positive Control: Mouse Liver.](http://images.novusbio.com/fullsize/PFKFB2-Antibody-Western-Blot-NBP1-79636-img0002.jpg "Western Blot: PFKFB2 Antibody [NBP1-79636] - Titration: 1.0 ug/ml Positive Control: Mouse Liver.")
![Western Blot: PFKFB2 Overexpression Lysate (Adult Normal) [NBL1-14311] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PFKFB2.](http://images.novusbio.com/fullsize/PFKFB2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14311-img0002.jpg "Western Blot: PFKFB2 Overexpression Lysate (Adult Normal) [NBL1-14311] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PFKFB2.")
![Western Blot: PFKFB2 [p Ser483] Antibody [NBP3-38640] - Western blot analysis of lysates from HeLa cells, using PFKFB2 Rabbit pAb at 1:2000 dilution. HeLa cells were treated by IGF-1 (50 ng/ml) at 37C for 30 minutes after serum-starvation overnight.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.](http://images.novusbio.com/fullsize/nbp3-38640_rabbit-pfkfb2-p-ser-483-pab-912202414112898.jpg "Western Blot: PFKFB2 [p Ser483] Antibody [NBP3-38640] - Western blot analysis of lysates from HeLa cells, using PFKFB2 Rabbit pAb at 1:2000 dilution. HeLa cells were treated by IGF-1 (50 ng/ml) at 37C for 30 minutes after serum-starvation overnight.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.")