PFKFB2 Antibody [DyLight 594]

• Reactivity: Hu, Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: DyLight 594

PFKFB2 Antibody [DyLight 594] Summary

• Immunogen: The immunogen this antibody was made to, maps to a region between residue 455 to 505 of human 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 using the numbering given in entry NP_006203.2 (GeneID 5208).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PFKFB2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for PFKFB2 Antibody [DyLight 594]

• 6PF-2-K/Fru-2,6-P2ase 2
• 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2
• 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2
• DKFZp781D2217
• fructose-2,6-bisphosphatase, cardiac isozyme
• MGC138308,6PF-2-K/Fru-2,6-P2ase heart-type isozyme
• MGC138310
• PFK/FBPase 2
• PFK-2/FBPase-2
• PFKFB, cardiac

Background

Phosphofructokinases (PFK) are regulatory glycolytic enzymes that convert fructose 6-phosphate and ATP into fructose 1,6-bisphosphate (through PFK-1), fructose 2,6-bisphosphate (through PFK-2) and ADP. Human PFK-1 is tetrameric and isoenzymes include, PFK-1 muscle (PFKM, PFK-A), PFK-1 liver (PFKL, PFK-B) and PFK-1 platelet (PFKP, PFK-C, PFKF). PFK-1 is inhibited by ATP and citrate (from the tricarboxylic acid cycle). PFK-1 undergoes activation in the presence of elevated AMP. The most potent activator is fructose 2,6-bisphosphate, which is produced by PFK-2 from the same substrate, fructose 6-phosphate. PFK-2 is bifunctional and a key regulator for PFK-1. PFK-2 catalyzes the synthesis of fructose 2,6-bisphosphate and contains fructose 2,6-biphosphatase activity that catalyzes the degradation of fructose 2,6-bisphosphate. PFK-2 is dimeric and isoenzymes include PFK-2 liver (PFKFB1, PFRX), PFK-2 cardiac (PFKFB2), PFK-2 placental (PFKFB3, inducible PFK-2) and PFK-2 testis (PFKFB4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PFKFB2