PFKFB1 Antibody

Images

 
Western Blot: PFKFB1 Antibody [H00005207-D01P] - Analysis of PFKFB1 expression in transfected 293T cell line by PFKFB1 polyclonal antibody.Lane 1: PFKFB1 transfected lysate(54.70 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: PFKFB1 Antibody [H00005207-D01P] - Analysis of antibody to PFKFB1 on HeLa cell. Antibody concentration 20 ug/ml.

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

PFKFB1 Antibody Summary

Description
Quality control test: Antibody reactive against mammalian transfected lysate.
Immunogen
PFKFB1 (NP_002616.1, 1 a.a. - 471 a.a.) full-length human protein. MSPEMGELTQTRLQKIWIPHSSGSSRLQRRRGSSIPQFTNSPTMVIMVGLPARGKTYISTKLTRYLNWIGTPTKVFNLGQYRREAVSYKNYEFFLPDNMEALQIRKQCALAALKDVHNYLSHEEGHVAVFDATNTTRERRSLILQFAKEHGYKVFFIESICNDPGIIAENIRQVKLGSPDYIDCDREKVLEDFLKRIECYEVNYQPLDEELDSHLSYIKIFDVGTRYMVNRVQDHIQSRTVYYLMNIHVTPRSIYLCRHGESELNIRGRIGGDSGLSVRGKQYAYALANFIQSQGISSLKVWTSRMKRTIQTAEALGVPYEQWKALNEIDAGVCEEMTYEEIQEHYPEEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVLVICHQAVMRCLLAYFLDKSSDELPYLKCPLHTVLKLTPVAYGCKVESIYLNVEAVNTHREKPENVDITREPEEALDTVPAHY
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PFKFB1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
Application Notes
It has been used for WB and Functional.
Publications
Read Publication using
H00005207-D01P in the following applications:

  • WB
    1 publication

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 28706006).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein G purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PFKFB1 Antibody

  • 2-kinase:fructose-2,6-bisphosphatase
  • 6PF-2-K/Fru-2,6-P2ase liver isozyme
  • 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1
  • F6PK
  • fructose-6-phosphate
  • MGC116715,6PF-2-K/Fru-2,6-P2ase 1
  • MGC116717
  • PFK/FBPase 1
  • PFRXHL2K

Background

Phosphofructokinases (PFK) are regulatory glycolytic enzymes that convert fructose 6-phosphate and ATP into fructose 1,6-bisphosphate (through PFK-1), fructose 2,6-bisphosphate (through PFK-2), and ADP. Human PFK-1 is tetrameric and isoenzymes include, PFK-1 muscle (PFKM, PFK-A), PFK-1 liver (PFKL, PFK-B), and PFK-1 platelet (PFKP, PFK-C, PFKF). PFK-1 is inhibited by ATP and citrate (from the tricarboxylic acid cycle). PFK-1 undergoes activation in the presence of elevated AMP. The most potent activator is fructose-2,6-bisphosphate, which is produced by PFK-2 from the same substrate, fructose 6-phosphate. PFK-2 is bifunctional and a key regulator for PFK-1. PFK-2 catalyzes the synthesis of fructose-2,6-bisphosphate, and contains fructose-2,6-biphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate. PFK-2 is dimeric and isoenzymes include PFK-2 liver (PFKFB1, PFRX), PFK-2 cardiac (PFKFB2), PFK-2 placental (PFKFB3, inducible PFK-2) and PFK-2 testis (PFKFB4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PFKFB1 Antibody (H00005207-D01P)(1)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: WB.


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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol PFKFB1
Uniprot