PFKFB1 Antibody - Azide and BSA Free

Western Blot: PFKFB1 Antibody [H00005207-D01P] - Analysis of PFKFB1 expression in transfected 293T cell line by PFKFB1 polyclonal antibody.Lane 1: PFKFB1 transfected lysate(54.70 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: PFKFB1 Antibody [H00005207-D01P] - Analysis of antibody to PFKFB1 on HeLa cell. Antibody concentration 20 ug/ml.

• Reactivity: Hu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PFKFB1 Antibody - Azide and BSA Free Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: PFKFB1 (NP_002616.1, 1 a.a. - 471 a.a.) full-length human protein. MSPEMGELTQTRLQKIWIPHSSGSSRLQRRRGSSIPQFTNSPTMVIMVGLPARGKTYISTKLTRYLNWIGTPTKVFNLGQYRREAVSYKNYEFFLPDNMEALQIRKQCALAALKDVHNYLSHEEGHVAVFDATNTTRERRSLILQFAKEHGYKVFFIESICNDPGIIAENIRQVKLGSPDYIDCDREKVLEDFLKRIECYEVNYQPLDEELDSHLSYIKIFDVGTRYMVNRVQDHIQSRTVYYLMNIHVTPRSIYLCRHGESELNIRGRIGGDSGLSVRGKQYAYALANFIQSQGISSLKVWTSRMKRTIQTAEALGVPYEQWKALNEIDAGVCEEMTYEEIQEHYPEEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVLVICHQAVMRCLLAYFLDKSSDELPYLKCPLHTVLKLTPVAYGCKVESIYLNVEAVNTHREKPENVDITREPEEALDTVPAHY
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PFKFB1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: It has been used for WB and Functional.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 28706006).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein G purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PFKFB1 Antibody - Azide and BSA Free

• 2-kinase:fructose-2,6-bisphosphatase
• 6PF-2-K/Fru-2,6-P2ase liver isozyme
• 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1
• F6PK
• fructose-6-phosphate
• MGC116715,6PF-2-K/Fru-2,6-P2ase 1
• MGC116717
• PFK/FBPase 1
• PFRXHL2K

Background

Phosphofructokinases (PFK) are regulatory glycolytic enzymes that convert fructose 6-phosphate and ATP into fructose 1,6-bisphosphate (through PFK-1), fructose 2,6-bisphosphate (through PFK-2), and ADP. Human PFK-1 is tetrameric and isoenzymes include, PFK-1 muscle (PFKM, PFK-A), PFK-1 liver (PFKL, PFK-B), and PFK-1 platelet (PFKP, PFK-C, PFKF). PFK-1 is inhibited by ATP and citrate (from the tricarboxylic acid cycle). PFK-1 undergoes activation in the presence of elevated AMP. The most potent activator is fructose-2,6-bisphosphate, which is produced by PFK-2 from the same substrate, fructose 6-phosphate. PFK-2 is bifunctional and a key regulator for PFK-1. PFK-2 catalyzes the synthesis of fructose-2,6-bisphosphate, and contains fructose-2,6-biphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate. PFK-2 is dimeric and isoenzymes include PFK-2 liver (PFKFB1, PFRX), PFK-2 cardiac (PFKFB2), PFK-2 placental (PFKFB3, inducible PFK-2) and PFK-2 testis (PFKFB4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PFKFB1 Antibody (H00005207-D01P)(1)

Publication using H00005207-D01P

• Gibb AA, Lorkiewicz PK, Zheng YT et al. Integration of flux measurements to resolve changes in anabolic and catabolic metabolism in cardiac myocytes Biochem. J. 2017-07-13 [PMID: 28706006] (WB, Rat)

Bioinformatics

• Gene Symbol: PFKFB1
• Uniprot:
  • NP_002616.1(Human)