Peroxiredoxin 4 Antibody - BSA Free

Western Blot: Peroxiredoxin 4 Antibody [NBP2-19778] - PRDX2 knockdown increases PRDX4 protein levels in HeLa cellsHeLa subclones were exposed to 20% or 1% O2 for indicated time. Each WCL was subject to immunoblot assays with the indicated antibodies. Image collected and cropped by CiteAb from the following publication (oncotarget.com/abstract/7142), licensed under a CC-BY license.

Immunohistochemistry-Paraffin: Peroxiredoxin-4 Antibody [NBP2-19778] - Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using antibody at 1:250 dilution.

Western Blot: Peroxiredoxin-4 Antibody [NBP2-19778] - Sample (30 ug of whole cell lysate) A: NT2D1 12% SDS PAGE gel, diluted at 1:1000.

Western Blot: Peroxiredoxin-4 Antibody [NBP2-19778] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.

Mapping the PRDX2 & PRDX4 binding domains of HIF-1 alpha A. & B. HeLa cells were transfected with PRDX2-V5 (A) or PRDX4-V5 (B) vector & WCL was incubated with purified GST or GST-HIF-1 alpha fusion protein in the presence of glutathione-Sepharose beads, followed by immunoblot assays with anti-V5 antibody (upper panels) or Ponceau S staining (lower panels). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.7142), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Hypoxia induces the nuclear translocation of PRDX2 & PRDX4HeLa cells were transfected with vector encoding PRDX2-V5 (P2) or PRDX4-V5 (P4), or empty vector (EV), & exposed to 20% or 1% O2 for 48 h. Nuclear & cytosolic fractions were isolated & subject to immunoblot assays with antibodies against HIF-1 alpha , HIF-2 alpha , V5, alpha -tubulin, & histone H3. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.7142), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Effect of PRDX2 & PRDX4 on HIF-1 alpha -p300 interactionHeLa cells were transfected with empty vector (EV) or vector encoding PRDX2-V5 or PRDX4-V5, & exposed to 1% O2 for 24 h. WCL was subject to IP with anti-p300 antibody, followed by immunoblot assays using antibodies against HIF-1 alpha , V5, & p300. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.7142), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Expression of PRDX2 or PRDX4 does not affect HIF-1 alpha or HIF-2 alpha protein levelsA. & B. HeLa cells were transfected with EV or vector encoding PRDX2-V5 (A, P2) or PRDX4-V5 (B, P4), & exposed to 20% or 1% O2 for 24 h. WCL was subject to immunoblot assays with antibody against HIF-1 alpha , HIF-2 alpha , V5, or acin. C. HeLa-shSC (sc) & HeLa-shPRDX(2+4) (2+4) cells were exposed to 20% or 1% O2 for 24 h in the presence of doxycycline. WCL was subject to immunoblot assays with antibodies against HIF-1 alpha , HIF-2 alpha , PRDX2, PRDX4, & actin. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.7142), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Peroxiredoxin 4 Antibody [NBP2-19778] - Expression of PRDX2 or PRDX4 does not affect HIF-1 alpha or HIF-2 alpha protein levelsA. & B. HeLa cells were transfected with EV or vector encoding PRDX2-V5 (A, P2) or PRDX4-V5 (B, P4), & exposed to 20% or 1% O2 for 24 h. WCL was subject to immunoblot assays with antibody against HIF-1 alpha , HIF-2 alpha , V5, or acin. C. HeLa-shSC (sc) & HeLa-shPRDX(2+4) (2+4) cells were exposed to 20% or 1% O2 for 24 h in the presence of doxycycline. WCL was subject to immunoblot assays with antibodies against HIF-1 alpha , HIF-2 alpha , PRDX2, PRDX4, & actin. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.7142), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, RM
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Peroxiredoxin 4 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Peroxiredoxin 4. The exact sequence is proprietary.
• Localization: Cytoplasm
• Predicted Species: Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRDX4
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 31 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Peroxiredoxin 4 Antibody - BSA Free

• Antioxidant enzyme AOE372
• AOE372
• AOE37-2PRX-4
• EC 1.11.1.15
• Peroxiredoxin 4
• Peroxiredoxin IV
• peroxiredoxin-4
• PRDX4
• Prx4
• prx-4
• prx-IV
• thioredoxin peroxidase (antioxidant enzyme)
• Thioredoxin peroxidase AO372
• Thioredoxin-dependent peroxide reductase A0372

Background

The protein encoded by this gene is an antioxidant enzyme and belongs to the peroxiredoxin family. The protein is localized to the cytoplasm. Peroxidases of the peroxiredoxin family reduce hydrogen peroxide and alkyl hydroperoxides to water and alcohol with the use of reducing equivalents derived from thiol-containing donor molecules. This protein has been found to play a regulatory role in the activation of the transcription factor NF-kappaB. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Peroxiredoxin 4 Antibody (NBP2-19778)(1)

Publication using NBP2-19778

• Luo W, Chen I, Chen Y et al. PRDX2 and PRDX4 are negative regulators of hypoxia-inducible factors under conditions of prolonged hypoxia Oncotarget. 2016-02-09 [PMID: 26837221] (WB)
  
  Details:
  The mechanism for feedback inhibition of hypoxia-inducible factors during prolonged hypoxia is clarified through studying the interaction of PRDX2 and PRDX4 with HIF-1 alpha and HIF-2 alpha.

Bioinformatics

• Gene Symbol: PRDX4
• Uniprot:
  • Q13162(Human)