Peripherin Overexpression Lysate (Native)


Western Blot: Peripherin Overexpression Lysate (Adult Normal) [NBL1-14825] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Peripherin.

Product Details

Applications WB
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Order Details

Peripherin Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC207561 and based on accession number NM_006262. The protein contains a C-terminal DDK tag.
Homo sapiens peripherin (PRPH), mRNA.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer


  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
53.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Lysate Details for Peripherin

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Peripherin Overexpression Lysate (Native)

  • neurofilament 4 (57kD)
  • Neurofilament 4
  • peripherin


This gene encodes a cytoskeletal protein found in neurons of the peripheral nervous system. The encoded protein is a type III intermediate filament protein with homology to other cytoskeletal proteins such as desmin, and is a different protein that the peripherin found in photoreceptors. Mutations in this gene have been associated with susceptibility to amyotrophic lateral sclerosis. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Av, Bv, Fi, Ma, Xp, Dr(-), In(-)
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
Species: Hu, Mu, Sh
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu
Applications: WB, ELISA, Flow
Species: Hu, Mu, Rt, Po, Bv
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Ca
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Rt, Po, Av, Bv, Ma
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Po, Av, Bv, Fe
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Fe, GP, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Rt, Po, Bv, Fe
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC-P
Species: Hu, Mu, Rt
Applications: WB, IHC
Species: Hu
Applications: ICC, ICFlow
Species: Hu, Rt, Pm
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP

Publications for Peripherin Lysate (NBL1-14825) (0)

There are no publications for Peripherin Lysate (NBL1-14825).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Peripherin Lysate (NBL1-14825) (0)

There are no reviews for Peripherin Lysate (NBL1-14825). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Peripherin Lysate (NBL1-14825) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Peripherin Products

Related Products by Gene

Bioinformatics Tool for Peripherin Lysate (NBL1-14825)

Discover related pathways, diseases and genes to Peripherin Lysate (NBL1-14825). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Peripherin Lysate (NBL1-14825)

Discover more about diseases related to Peripherin Lysate (NBL1-14825).

Pathways for Peripherin Lysate (NBL1-14825)

View related products by pathway.

PTMs for Peripherin Lysate (NBL1-14825)

Learn more about PTMs related to Peripherin Lysate (NBL1-14825).

Research Areas for Peripherin Lysate (NBL1-14825)

Find related products by research area.

Blogs on Peripherin.

A Look at Peripherin: The Unknown Filament
The exact function of Peripherin, or Neurofilament 4, is unknown however it has been suggested to play a role in axon formation and determining and maintaining the shape of nerve cells. Peripherin is a 470 amino acid Class-III neuronal intermediate fi...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol PRPH

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NBL1-14825 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought

GFAP Antibody
GFAP Antibody
GFAP Antibody