Perilipin-2/ADFP Antibody (788616) [Unconjugated]

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and Huh-7 human hepatoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Perilipin-2 Monoclonal Antibody (Catalog # MAB76341) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Perilipin-2 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Perilipin-2 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Perilipin-2 Monoclonal Antibody (Catalog # MAB76341) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Perilipin-2 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Perilipin-2 Monoclonal Antibody (Catalog # MAB76341) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: WB, IHC, ICC/IF
• Clone: 788616
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Perilipin-2/ADFP Antibody (788616) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Perilipin-2
  Met1-His437
  Accession # Q99541
• Specificity: Detects human Perilipin-2 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 5-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Perilipin-2/ADFP Antibody (788616) [Unconjugated]

• ADFP
• Adipophilin
• Adipose differentiation-related proteinadipophilin
• ADRP
• MGC10598
• perilipin 2
• Perilipin2
• Perilipin-2
• PLIN2

Background

Perilipin-2 (PLIN2), also known as Adipophilin (ADFP) and Adipose Differentiation-Related Protein (ADRP), is a widely expressed 52 kDa acylated protein that associates with membranes and cytoplasmic lipid droplets. It is upregulated during adipocyte differentiation and is a major component of milk fat globules. Perilipin-2 promotes the cellular uptake and accumulation of long chain fatty acids and regulates the intracellular distribution of phospholipids. It promotes hepatic insulin resistance in response to a high fat diet. Human Perilipin-2 shares 82% and 79% aa sequence identity with mouse and rat Perilipin-2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Perilipin-2/ADFP Antibody (MAB76341)(1)

Publication using MAB76341

• C Tonini, M Colardo, B Colella, S Di Bartolo, F Berardinel, G Caretti, V Pallottini, M Segatto Inhibition of Bromodomain and Extraterminal Domain (BET) Proteins by JQ1 Unravels a Novel Epigenetic Modulation to Control Lipid Homeostasis Int J Mol Sci, 2020-02-14;21(4):. 2020-02-14 [PMID: 32075110] (ICC/IF, Human)

Bioinformatics

• Uniprot:
  • Q99541(Human)