Perilipin-2/ADFP Antibody (2C5A3)

Western Blot: Perilipin-2/ADFP Antibody (2C5A3) [NBP2-23486] - Analysis of ADFP in (1) HEK293 and (2) PLIN2 hIgGFc transfected HEK293 cell lysate.

Immunocytochemistry/Immunofluorescence: Perilipin-2/ADFP Antibody (2C5A3) [NBP2-23486] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-ADFP/Perilipin-2 (2C5A3) NBP2-23486 at a 1:50 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was counterstained using Phalloidin at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry: Perilipin-2/ADFP Antibody (2C5A3) [NBP2-23486] - Analysis of ADFP in paraffin-embedded human esophageal cancer tissues with DAB staining.

Western Blot: Perilipin-2/ADFP Antibody (2C5A3) [NBP2-23486] - Analysis of ADFP in human ADFP recombinant protein. (Expected MW is 42.6 kDa)

Immunohistochemistry: Perilipin-2/ADFP Antibody (2C5A3) [NBP2-23486] - Analysis of ADFP in paraffin-embedded human liver cancer tissues with DAB staining.

Product Discontinued

Perilipin-2/ADFP Antibody (2C5A3) Summary

• Immunogen: Partial recombinant human ADFP (between residues 200-437) expressed in E. coli [UniProt Q99541]
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PLIN2
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:100
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 0.5% proprietary protein stabilizer
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Ammonium sulfate precipitation

Alternate Names for Perilipin-2/ADFP Antibody (2C5A3)

• ADFP
• Adipophilin
• Adipose differentiation-related proteinadipophilin
• ADRP
• MGC10598
• perilipin 2
• Perilipin2
• Perilipin-2
• PLIN2

Background

Perilipin-2/Plin-2, also known as adipose differentiation-related protein (ADFP), ADRP, and adipophilin, is a member of the perilipin family of lipid droplet (LD) proteins that functions in lipid accumulation, lipid storage, and LD formation (1,2). Perilipin-2 is ubiquitously expressed and is the major LD protein of the liver (1-3). Human perilipin-2 is 437 amino acids (aa) in length with a theoretical molecular weight of 48 kDa (1,4). Human and mouse perilipin-2 share 81.8% sequence identity. One defining structural feature of perilipin-2 and other LD-related proteins is formation of amphipathic alpha helices (1,3,5). The perilipin-2 protein contains a PAT (perilipin A, ADRP, TIP47) domain, a 11-mer repeat motif, and a 4-helix bundle (1,5). The PAT domain has a role in triglyceride (TG) stabilization, the 11-mer repeat motif functions in cytoplasmic LD binding, and the 4-helix bundle facilitates membrane binding (5). Unlike LDs coated with other perilipin family members (e.g., perilipin-1 and perilipin-5), LDs coated with perilipin-2 are more permissive to lipolysis and protective of lipids and TGs (1-3). Studies have revealed that perilipin-2 knockout (KO) in mice is associated with resistance to fatty liver disease and diet-induced obesity (1). On the other hand, overexpression of perilipin-2 causes increased lipid and TG accumulation, which is a feature of many metabolic, cardiovascular, and age-related diseases (2). Additional research has found higher levels of perilipin-2 is associated with certain cancers such as colorectal cancer and renal carcinomas, and it may be a useful biomarker for detection (2). Though more research is needed, it is possible that inhibition or blocking of perilipin-2 may be a key strategy to combatting several pathologies (2).

References

1. Itabe H, Yamaguchi T, Nimura S, Sasabe N. Perilipins: a diversity of intracellular lipid droplet proteins. Lipids Health Dis. 2017;16(1):83. https://doi.org/10.1186/s12944-017-0473-y

2. Conte M, Franceschi C, Sandri M, Salvioli S. Perilipin 2 and Age-Related Metabolic Diseases: A New Perspective. Trends Endocrinol Metab. 2016;27(12):893-903. https://doi.org/10.1016/j.tem.2016.09.001

3. Sztalryd C, Brasaemle DL. The perilipin family of lipid droplet proteins: Gatekeepers of intracellular lipolysis. Biochim Biophys Acta Mol Cell Biol Lipids. 2017;1862(10 Pt B):1221-1232. https://doi.org/10.1016/j.bbalip.2017.07.009

4. Uniprot (Q99541)

5. Chong BM, Reigan P, Mayle-Combs KD, Orlicky DJ, McManaman JL. Determinants of adipophilin function in milk lipid formation and secretion. Trends Endocrinol Metab. 2011;22(6):211-217. https://10.1016/j.tem.2011.04.003

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Perilipin-2/ADFP Antibody (NBP2-23486)(1)

Publication using NBP2-23486

• Wang R, Nissen NN, Zhang Y Et al. Circulating Fatty Objects and Their Preferential Presence in Pancreatic Cancer Patient Blood Samples Front Physiol 2022-03-03 [PMID: 35237181] (Human)
  
  Details:
  Citation using the Biotin version of this antibody.

Bioinformatics

• Gene Symbol: PLIN2
• Entrez:
  • 123(Human)
• Uniprot:
  • Q99541(Human)