PELO Antibody - BSA Free

Western Blot: PELO Antibody [NBP2-19769] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PELO antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: PELO Antibody [NBP2-19769] - Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PELO protein stained by PELO antibody diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: PELO Antibody [NBP2-19769] - Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using antibody at 1:500 dilution.

Western Blot: PELO Antibody [NBP2-19769] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PELO antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: PELO Antibody [NBP2-19769] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PELO antibody (NBP2-19769) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Bv, Ch, RM
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PELO Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PELO Antibody - BSA Free (NBP2-19769) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-PELO Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human PELO. The exact sequence is proprietary.
• Localization: Nucleus (Potential); Cytoplasm
• Predicted Species: Bovine (98%), Rhesus Macaque (100%), Chicken (95%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PELO
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 43 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Zebrafish (82%), Xenopus laevis (83%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for PELO Antibody - BSA Free

• EC 3.1
• pelota (Drosophila) homolog
• pelota homolog (Drosophila)
• PRO1770
• protein pelota homolog

Background

This gene encodes a protein which contains a conserved nuclear localization signal. The encoded protein may have a role in spermatogenesis, cell cycle control, and in meiotic cell division. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for PELO Antibody (NBP2-19769)(1)

Publication using NBP2-19769

• Ghiasi SM, Krogh N, Tyrberg B et al. The No-Go and Nonsense-Mediated RNA Decay Pathways are Regulated by Inflammatory Cytokines in Insulin-Producing Cells and Human Islets and Determine beta-Cell Insulin Biosynthesis and Survival Diabetes 2018-07-31 [PMID: 30065031] (WB, Rat, Human)

Bioinformatics

• Gene Symbol: PELO
• Uniprot:
  • Q9BRX2(Human)