SRPX Antibody - BSA Free

Western Blot: SRPX Antibody [NBP1-77086] - SK-N-SH cell lysate with SRPX antibody at 0.25 ug/mL in (A) the absence and (B) the presence of blocking peptide.

Immunocytochemistry/Immunofluorescence: SRPX Antibody [NBP1-77086] - Immunofluorescence of SRPX1 in human brain tissue with SRPX1 antibody at 20 ug/mL.

Immunohistochemistry: SRPX Antibody - BSA Free [NBP1-77086] - Immunohistochemistry of SRPX in human brain tissue with SRPX antibody at 5 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

SRPX Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a 13 amino acid synthetic peptide from near the amino terminus of human SRPX1. The immunogen is located within amino acids 30 - 80 of SRPX1. Amino Acid Squence: VGYSHPRYKDTPW
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SRPX
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunohistochemistry 5 ug/mL
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 0.25 ug/ml
• Control Peptide:

  SRPX Peptide (NBP1-77086PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for SRPX Antibody - BSA Free

• DRS
• ETX1SRPX1
• sushi repeat-containing protein SRPX
• sushi-repeat containing protein, X-linked
• sushi-repeat-containing protein, X chromosome
• sushi-repeat-containing protein, X-linked

Background

SRPX1 was initially identified as a candidate gene for X-linked retinitis pigmentosa and is overexpressed in the trabecular network of the eye in glaucoma conditions. Its expression is significantly downregulated in a number cancer cell lines and malignant tumor tissues and its ectopic expression induces apoptosis through the endoplasmic reticulum and the activation of caspase-12, -9, and -3, indicating that it can function as a tumor suppressor. SRPX1 is also involved with the regulation of autophagy under low serum conditions and can associate with Rab24, a member of the Rab GTPase protein family that is involved in autophagy. This association of SRPX1 with Rab24 is enhanced during autophagy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SRPX Antibody (NBP1-77086)(1)

Publication using NBP1-77086

• Ampudia-Mesias E, El-Hadad S, Cameron CS et al. SRPX Emerges as a Potential Tumor Marker in the Extracellular Vesicles of Glioblastoma Cancers 2022-04-14 [PMID: 35454889] (IF/IHC, Human)

Bioinformatics

• Gene Symbol: SRPX
• Uniprot:
  • NP_006298(Human)