Pellino 2 Antibody - BSA Free

Western Blot: Pellino 2 Antibody [NBP2-33679] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4

Immunocytochemistry/Immunofluorescence: Pellino 2 Antibody [NBP2-33679] - Staining of human cell line U-2 OS shows localization to nucleus, nucleoli & intermediate filaments. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: Pellino 2 Antibody [NBP2-33679] - Staining of human stomach shows moderate nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: Pellino 2 Antibody [NBP2-33679] - Staining of human kidney shows moderate nuclear positivity in cells in glomeruli.

Immunohistochemistry-Paraffin: Pellino 2 Antibody [NBP2-33679] - Staining of human nasopharynx shows strong positivity in respiratory epithelial cells.

Immunohistochemistry-Paraffin: Pellino 2 Antibody [NBP2-33679] - Staining of human pancreas shows weak nuclear positivity in exocrine glandular cells.

Immunohistochemistry-Paraffin: Pellino 2 Antibody [NBP2-33679] - Staining of human placenta shows weak nuclear positivity in trophoblastic cells.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Pellino 2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Pellino 2 Antibody - BSA Free (NBP2-33679) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: NRKEVVEEKQPWAYLSCGHVHGYHN
• Predicted Species: Mouse (96%), Rat (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PELI2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  Pellino 2 Protein (NBP2-33679PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Pellino 2 Antibody - BSA Free

• PELI2
• pellino (Drosophila) homolog 2
• pellino homolog 2 (Drosophila)
• pellino-2
• protein pellino homolog 2

Background

Scaffold protein which probably links Toll-like receptors (TLRs) to basic cellular processes via itsinteraction with the complex containing IRAK kinases and TRAF6. Can activate the MAP (mitogen activated protein)kinase pathway leading to activation of ELK1. Not required for NF-kappa-B activation

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PELI2
• Uniprot:
  • Q9HAT8(Human)