PDZK1 Antibody [Unconjugated]

Western blot shows lysates of mouse liver tissue and rat liver tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat PDZK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5780) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PDZK1 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

PDZK1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse PDZK1
  aa 1-519
  Accession # Q9JIL4
• Specificity: Detects mouse and rat PDZK1 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PDZK1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PDZK1 Antibody [Unconjugated]

• CAP70
• CFTR-associated protein of 70 kDa
• CLAMP
• Na(+)/H(+) exchanger regulatory factor 3
• Na/Pi cotransporter C-terminal-associated protein 1
• NaPi-Cap1
• NHERF3
• NHERF-3
• NHERF3PDZD1Na(+)/H(+) exchange regulatory cofactor NHE-RF3
• PDZ domain containing 1
• PDZ domain-containing protein 1
• PDZ-containing kidney protein 1
• PDZD1
• PDZK1
• Sodium-hydrogen exchanger regulatory factor 3

Background

PDZK1 (PDZ Domain-containing 1; also CFTR-associated protein of 70 kDa/CAP70) is a 65‑70 kDa member of the NHER (Na/H Exchange Regulator) family of proteins. It is only expressed in epithelium, particularly that found in intestine, kidney and liver. PDZK1 serves as a scaffold protein that connects cell membrane proteins to cytoplasmic regulators. It also serves a critical role in basal-level bicarbonate secretion. Mouse PDZK1 is 519 amino acids (aa) in length and contains four PDZ domains (aa 9‑90; 128‑215; 243‑323; and 378‑458) plus three phosphorylation sites at Ser489, Ser492 and Ser514. Mouse PDZK1 shares 78% and 91% aa identity with human and rat PDZK1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PDZK1
• Uniprot:
  • Q9JIL4()