PDX-1/IPF1 Antibody (267712) [Allophycocyanin]

beta TC-6 mouse beta cell insulinoma cell line was stained with Mouse Anti-Human/Mouse PDX-1/IPF1 APC-conjugated Monoclonal Antibody (Catalog # IC2419A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu, Mu
• Applications: Flow
• Clone: 267712
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

PDX-1/IPF1 Antibody (267712) [Allophycocyanin] Summary

• Immunogen: E. coli-derived recombinant human PDX-1
  Ala91-Arg283
  Accession # P52945
• Specificity: Detects human and mouse PDX-1 in Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PDX1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PDX-1/IPF1 Antibody (267712) [Allophycocyanin]

• Glucose-sensitive factor
• IDX1
• IDX-1GSF
• Insulin promoter factor 1
• insulin promoter factor 1, homeodomain transcription factor
• Insulin upstream factor 1
• IPF1
• IPF1pancreas/duodenum homeobox protein 1
• Islet/duodenum homeobox-1
• MODY4IUF1
• pancreatic and duodenal homeobox 1
• pancreatic-duodenal homeobox factor 1
• PDX-1
• PDX-1IPF-1
• somatostatin transcription factor 1
• Somatostatin-transactivating factor 1
• STF-1IUF-1

Background

PDX-1 (Pancreatic-duodenal Homeobox Factor-1), also known as IDX-1 and STF-1 is a 31 kDa member of the IPF-1 (Insulin Promoter Factor) subfamily, Antp (antennapedia) family of molecules. Human PDX-1 is 283 amino acids (aa) in length, and although its predicted MW is approximately 30 kDa, it runs anomalously in SDS-PAGE at 40-46 kDa. PDX-1 possesses one transctivation domain (aa 13-73) and one DNA-binding homeodomain (aa 146-205) that contains an NLS between aa 197-203. Phosphorylation is known to occur on Ser61 and Ser269, and methylation is found on Lys131. PDX-1 drives the development of the embryonic pancreas, and post-natally it is found in both beta - and δ (somatostatin) islet cells. This transcription factor complexes with multiple cofactors (Set9; NeuroD1; MafA; HMGA1) in its regulation of beta -cell division and insulin synthesis, and serves principally as an RNA pol II binding modulator. Over aa 91-283, human and mouse PDX-1 share 87% aa sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PDX1
• Uniprot:
  • P52945()