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PDLIM4 Antibody

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Immunocytochemistry/Immunofluorescence: PDLIM4 Antibody [NBP2-98063] - Immunofluorescence staining of PDLIM4 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human PDLIM4 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.
Immunocytochemistry/ Immunofluorescence: PDLIM4 Antibody [NBP2-98063] - Immunofluorescence staining of PDLIM4 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% ...read more
Immunohistochemistry-Paraffin: PDLIM4 Antibody [NBP2-98063] - Immunochemical staining of human PDLIM4 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-98063AF350
Alexa Fluor 405
NBP2-98063AF405
Alexa Fluor 488
NBP2-98063AF488
Alexa Fluor 532
NBP2-98063AF532
Alexa Fluor 594
NBP2-98063AF594
Alexa Fluor 647
NBP2-98063AF647
Alexa Fluor 700
NBP2-98063AF700
Alexa Fluor 750
NBP2-98063AF750
Biotin
NBP2-98063B
DyLight 350
NBP2-98063UV
DyLight 405
NBP2-98063V
DyLight 488
NBP2-98063G
DyLight 550
NBP2-98063R
DyLight 594
NBP2-98063DL594
DyLight 650
NBP2-98063C
DyLight 680
NBP2-98063FR
DyLight 755
NBP2-98063IR
FITC
NBP2-98063F
HRP
NBP2-98063H
Janelia Fluor 549
NBP2-98063JF549
Janelia Fluor 646
NBP2-98063JF646
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PDLIM4 Antibody Summary

Immunogen
Produced in rabbits immunized with E. coli-derived Human PDLIM4 fragment.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PDLIM4
Purity
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0)
Preservative
0.03% Proclin 300
Purity
Antigen Affinity-purified

Notes

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Alternate Names for PDLIM4 Antibody

  • LIM domain protein
  • LIM protein RIL
  • PDZ and LIM domain 4
  • PDZ and LIM domain protein 4
  • Reversion-induced LIM protein
  • RILenigma homolog

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: PDLIM5 Antibody [NBP1-84843] - Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane, cytosol & focal adhesion sites.
Immunohistochemistry-Paraffin: PDLIM5 Antibody [NBP1-84843] - Staining of human heart muscle shows strong cytoplasmic positivity in cardiomyocytes.
Immunohistochemistry-Paraffin: PDLIM5 Antibody [NBP1-84843] - Staining in human heart muscle and testis tissues.. Corresponding PDLIM5 RNA-seq data are presented for the same tissues.
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Western Blot: PDLIM1 Antibody [NBP1-84443] - Analysis in human cell line CACO-2 and human cell line U-251 MG.
Immunohistochemistry-Paraffin: PDLIM1 Antibody [NBP1-84443] - Staining of human Heart muscle shows moderate cytoplasmic positivity in cardiomyocytes.
Immunohistochemistry-Paraffin: PDLIM1 Antibody [NBP1-84443] - Staining of human Pancreas shows strong cytoplasmic positivity in islets of Langerhans.
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Immunocytochemistry/Immunofluorescence: Zyxin Antibody (2C10-4A7) [H00007791-M01] - Analysis of monoclonal antibody to ZYX on HeLa cell . Antibody concentration 10 ug/ml.
Immunoprecipitation: Zyxin Antibody (2C10-4A7) [H00007791-M01] - Analysis of ZYX transfected lysate using anti-ZYX monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ZYX MaxPab rabbit polyclonal antibody.
Western Blot: Zyxin Antibody (2C10-4A7) [H00007791-M01] - Analysis of ZYX over-expressed 293 cell line, cotransfected with ZYX Validated Chimera RNAi ( Cat # H00007791-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ZYX monoclonal antibody (M01), clone 2C10-4A7 (Cat # H00007791-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
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Immunohistochemistry-Paraffin: FHL2 Antibody [NBP1-84978] - Analysis in human heart muscle and liver tissues. Corresponding FHL2 RNA-seq data are presented for the same tissues.
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NBP2-70739
Western Blot: FHL1 Antibody (OTI3G7) - Azide and BSA Free [NBP2-70739] - Analysis of extracts (35ug) from 9 different cell lines by using anti-FHL1 monoclonal antibody.
Western Blot: FHL1 Antibody (OTI3G7) - Azide and BSA Free [NBP2-70739] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FHL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FHL1.
Flow Cytometry: FHL1 Antibody (OTI3G7) - Azide and BSA Free [NBP2-70739] - Analysis of Hela cells, using anti-FHL1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Species: Hu, Mu, Rt
Applications: CyTOF-ready, Flow, ICC/IF, WB
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Immunocytochemistry/Immunofluorescence: LPP Antibody [NBP1-89549] - Staining of human cell line U-251 MG shows localization to cytosol and focal adhesion sites. Antibody staining is shown in green.
Species: Hu, Mu, Rt
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Western Blot: LDB3 Antibody (3C8) [H00011155-M06] - Western blot analysis of LDB3 over-expressed 293 cell line, cotransfected with LDB3 Validated Chimera RNAi or non-transfected control. Blot probed with H00011155-M06. GAPDH (36.1 kDa) used as loading control.
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NBP1-58268
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H00008458-M06
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Western Blot: Alkaline Phosphatase/ALPP Antibody (8B6) [NB110-3638] - Analysis of Alkaline Phosphatase (Placental) expression in JAR whole cell lysate.
(1 Review)
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Immunohistochemistry: ATRNL1 Antibody [NBP1-89131] - Staining of human small intestine shows strong cytoplasmic and membranous positivity.
Immunocytochemistry/Immunofluorescence: ATRNL1 Antibody [NBP1-89131] - Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
Immunohistochemistry-Paraffin: ATRNL1 Antibody [NBP1-89131] - Staining of human small intestine shows strong cytoplasmic and membranous positivity.
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Western Blot: NAT10 Antibody [NBP2-32263] - Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-NAT10 antibody NBP2-32263 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: NAT10 Antibody [NBP2-32263] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from 293T cells. Antibodies: Affinity purified rabbit anti-NAT10 antibody NBP2-32263 used for IP at 6 ug per reaction. NAT10 was also immunoprecipitated by rabbit anti-NAT10 antibody BL15900. For blotting immunoprecipitated NAT10, NBP2-32263 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
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NB100-56139
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Immunohistochemistry-Paraffin: PTPN13/PTPL1 Antibody - Unpurified [NB100-56139] - PTPN13/PTPL1 Antibody [NB100-56139] - Analysis of PTPN13/PTPL1 in cell lines using this antibody at 1:2000. In ovarian carcinoma cell lines PTPN13/PTPL1, expression was detected in SK-OV-3 and OVCAR-3, but not in BG-1 or HEY. Human 293 kidney and Jurkat T cell lines were used as positive and negative controls, respectively.
Western Blot: PTPN13/PTPL1 Antibody - Unpurified [NB100-56139] - PTPN13/PTPL1 Antibody [NB100-56139] - Analysis of PTPN13/PTPL1 using this antibody. In ovarian carcinoma cell lines PTPN13/PTPL1 expression was detected in SK-OV-3 and OVCAR-3, but not in BG-1 or HEY. Human Jurkat T and 293 kidney cell lines were used as negative and positive controls, respectively.
(1 Review)
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PDLIM4 Antibody
NBP2-98063
PDLIM4 Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P

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Bioinformatics

Gene Symbol PDLIM4