PDLIM2 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: PDLIM2 Antibody [NBP1-85800] - Immunofluorescent staining of human cell line U-2 OS shows localization to actin filaments & focal adhesion sites.

Staining of human placenta shows strong membranous and cytoplasmic positivity in trophoblastic cells.

Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 17Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PDLIM2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PDLIM2 Antibody - BSA Free (NBP1-85800) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: SPGHTNGDSSLEVLATRFQGSVRTYTESQSSLRSSYSSPTSLSPRAGSPFSPPPSSSSLTGEAAISRSFQSLACSPGLPAADRLSYSGRPGSRQAGLGRAGDSAVLVLPPSPGPRSSRPSMDSEG
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PDLIM2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  PDLIM2 Protein (NBP1-85800PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for PDLIM2 Antibody - BSA Free

• FLJ34715
• MYSTIQUE
• PDZ and LIM domain 2 (mystique)
• PDZ and LIM domain protein 2
• PDZ-LIM protein mystique
• SLIM

Background

Probable adapter protein located at the actin cytoskeleton that promotes cell attachment. Necessary for themigratory capacity of epithelial cells. Overexpression enhances cell adhesion to collagen and fibronectin andsuppresses anchorage independent growth. May contribute to tumor cell migratory capacity

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PDLIM2