PDHX Antibody - BSA Free

Western Blot: PDHX Antibody [NBP1-84080] - Analysis using Anti-PDHX antibody NBP1-84080 (A) shows similar pattern to independent antibody NBP1-84079 (B).

Staining of human heart muscle, kidney, pancreas and skeletal muscle using Anti-PDHX antibody NBP1-84080 (A) shows similar protein distribution across tissues to independent antibody NBP1-84079 (B).

Staining of human heart muscle shows strong granular cytoplasmic positivity in cardiomyocytes.

Staining of human skeletal muscle shows moderate granular cytolasmic positivity in myocytes.

Staining of human pancreas shows weak granular cytoplasmic positivity in exocrine glandular cells.

Analysis in human heart muscle and pancreas tissues using NBP1-84080 antibody. Corresponding PDHX RNA-seq data are presented for the same tissues.

Staining of human kidney shows moderate granular cytoplasmic positivity in cells) in tubules.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PDHX Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: PAARNILEKHSLDASQGTATGPRGIFTKEDALKLVQLKQTGKITESRPTPAPTATPTAPSPLQATAGPSYPRPVIPPVSTPGQPNAVGTFTEI
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PDHX
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  PDHX Protein (NBP1-84080PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%), Rat (80%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for PDHX Antibody - BSA Free

• Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenasecomplex
• DLDBP
• E3-binding protein
• E3BP
• E3BP1
• E3BPpyruvate dehydrogenase complex, E3-binding protein subunit
• OPDX
• PDHX
• PDX1
• PDX1Lipoyl-containing pyruvate dehydrogenase complex component X
• proX
• proXpyruvate dehydrogenase complex, lipoyl-containing component X
• pyruvate dehydrogenase complex, component X
• pyruvate dehydrogenase protein X component, mitochondrial

Background

The PDHX gene encodes component X of the pyruvate dehydrogenase (PDH) complex. For a detailed description of the pyruvate dehydrogenase complex, see MIM 300502. The mammalian PDH complex differs from that in E. coli and from the other mammalian alpha-keto acid dehydrogenases by the presence of a 53-kD protein called protein X. Component X binds to the E3 (MIM 238331) component of the PDH complex (Robinson et al., 1990; Aral et al., 1997).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PDHX