PDHX Antibody [Unconjugated]

Images

 
Western blot shows lysates of 293T human embryonic kidney cell line, mouse heart tissue, and rat heart tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat PDHX Polyclonal Antibody (Catalog # ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price

PDHX Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human PDHX
aa 387-501
Accession # P15374
Specificity
Detects human, mouse, and rat PDHX in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PDHX
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PDHX Antibody [Unconjugated]

  • Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenasecomplex
  • DLDBP
  • E3-binding protein
  • E3BP
  • E3BP1
  • E3BPpyruvate dehydrogenase complex, E3-binding protein subunit
  • OPDX
  • PDHX
  • PDX1
  • PDX1Lipoyl-containing pyruvate dehydrogenase complex component X
  • proX
  • proXpyruvate dehydrogenase complex, lipoyl-containing component X
  • pyruvate dehydrogenase complex, component X
  • pyruvate dehydrogenase protein X component, mitochondrial

Background

UCH-L3 (Ubiquitin carboxyterminal hydrolase isozyme 3) is a 26‑28 kDa member of the peptidase C12 family of enzymes. It shows wide expression, being noted in adipocytes, renal duct epithelium, neurons, and striated muscle. UCH-L3 cleaves both monomeric ubiquitin (Ub) from Ub-protein conjugates, and a GGLRQ peptide from the C-terminus of the Ub-like protein NEDD-8. Notably, UCH-L3 activity is muted in the presence of Ub interacting dimers. Human UCH-L3 is 230 amino acids (aa) in length. It is phosphorylated on Ser75 and Ser130, and contains two ubiquitin-binding sequences between aa 40‑57 and 178‑186. Four potential splice forms have been reported. Two show a four aa deletion between aa 15‑18, with one also containing a 14 aa substitution for aa 179 - 230. Two others contain an alternative start site at Met37, one of which is also accompanied by a seven aa substitution for aa 143‑230. Full-length human UCH-L3 shares 98% aa identity with mouse and rat UCH-L3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
AF3444
Species: Hu
Applications: WB
NBP1-49672
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-05189
Species: Hu, Ma
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP1-31302
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-22218
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
AF2400
Species: Hu
Applications: WB, ChIP, ICC
NBP1-89522
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
AF2614
Species: Hu
Applications: WB, ICC
H00004760-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC-P
NBP1-89150
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
AF2574
Species: Hu
Applications: CyTOF-ready, ICFlow
H00256297-M05
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NB300-750
Species: Hu, Mu, Rt, Mk, Pm, Sh
Applications: WB, ICC/IF, IP, ICC
NBP2-27079
Species: Hu, Ca, Pm
Applications: WB, IHC-P
NB100-64650
Species: Hu, Mu, Rt, Po, GP, Ze
Applications: IHC, IHC-Fr, IHC-P
NBP1-89680
Species: Hu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NBP1-47778
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-82554
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-88347
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P

Publications for PDHX Antibody (AF6014) (0)

There are no publications for PDHX Antibody (AF6014).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for PDHX Antibody (AF6014) (0)

There are no reviews for PDHX Antibody (AF6014). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for PDHX Antibody (AF6014) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for PDHX Antibody (AF6014)

Discover related pathways, diseases and genes to PDHX Antibody (AF6014). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for PDHX Antibody (AF6014)

Discover more about diseases related to PDHX Antibody (AF6014).
 

Pathways for PDHX Antibody (AF6014)

View related products by pathway.

PTMs for PDHX Antibody (AF6014)

Learn more about PTMs related to PDHX Antibody (AF6014).

Blogs on PDHX

There are no specific blogs for PDHX, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our PDHX Antibody [Unconjugated] and receive a gift card or discount.

Bioinformatics

Gene Symbol PDHX
Uniprot