PDHX Antibody [Alexa Fluor® 350]

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 350

PDHX Antibody [Alexa Fluor® 350] Summary

• Immunogen: E. coli-derived recombinant human PDHX
  aa 387-501
  Accession # P15374
• Specificity: Detects human, mouse, and rat PDHX in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PDHX Antibody [Alexa Fluor® 350]

• Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenasecomplex
• DLDBP
• E3-binding protein
• E3BP
• E3BP1
• E3BPpyruvate dehydrogenase complex, E3-binding protein subunit
• OPDX
• PDHX
• PDX1
• PDX1Lipoyl-containing pyruvate dehydrogenase complex component X
• proX
• proXpyruvate dehydrogenase complex, lipoyl-containing component X
• pyruvate dehydrogenase complex, component X
• pyruvate dehydrogenase protein X component, mitochondrial

Background

UCH-L3 (Ubiquitin carboxyterminal hydrolase isozyme 3) is a 26‑28 kDa member of the peptidase C12 family of enzymes. It shows wide expression, being noted in adipocytes, renal duct epithelium, neurons, and striated muscle. UCH-L3 cleaves both monomeric ubiquitin (Ub) from Ub-protein conjugates, and a GGLRQ peptide from the C-terminus of the Ub-like protein NEDD-8. Notably, UCH-L3 activity is muted in the presence of Ub interacting dimers. Human UCH-L3 is 230 amino acids (aa) in length. It is phosphorylated on Ser75 and Ser130, and contains two ubiquitin-binding sequences between aa 40‑57 and 178‑186. Four potential splice forms have been reported. Two show a four aa deletion between aa 15‑18, with one also containing a 14 aa substitution for aa 179 - 230. Two others contain an alternative start site at Met37, one of which is also accompanied by a seven aa substitution for aa 143‑230. Full-length human UCH-L3 shares 98% aa identity with mouse and rat UCH-L3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.