PDGF R beta Antibody (APB5) - BSA Free

Flow Cytometry: PDGF R beta Antibody (APB5) [NBP1-43349] - Mouse brain pericytes - IgG (left) and PDGFRB (right). Image from verified customer review.

Flow Cytometry: PDGF R beta Antibody (APB5) [NBP1-43349] - Staining of NIH/3T3 cell line with 0.5 ug of Rat IgG2a kappa Isotype Control Purified (cat. 14-4321) (open histogram) or 0.5 ug of Anti-Mouse CD140b (PDGF Receptor b) Purified (filled histogram) followed by Anti-Rat IgG Biotin (cat. 13-4813) and Streptavidin PE (cat. 12-4317). Total viable cells were used for analysis.

Flow Cytometry: PDGF R beta Antibody (APB5) [NBP1-43349] - Analysis using the Biotin conjugate of NBP1-43349. Staining of NIH/3T3 cell line with 0.5 ug of Anti-Mouse CD140b (PDGF Receptor b) Biotin followed by Streptavidin PE. Appropriate isotype controls were used (open histogram).

• Reactivity: Mu
• Applications: Flow, IHC, MA
• Clone: APB5
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

PDGF R beta Antibody (APB5) - BSA Free Summary

• Immunogen: The immunogen for this antibody was PDGF receptor beta.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: PDGFRB
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry < 1.0 ug/10^6 cells
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Protein Array
• Application Notes: The APB5 antibody has been tested by flow cytometric analysis of NIH-3T3 cells. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Use in Protein Array reported in secitific publication PMID: 32499572

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for PDGF R beta Antibody (APB5) - BSA Free

• beta-type platelet-derived growth factor receptor
• CD140 antigen-like family member B
• CD140b antigen
• CD140b
• EC 2.7.10
• EC 2.7.10.1
• JTK12
• PDGF R beta
• PDGFR
• PDGFR1
• PDGFRB
• PDGF-R-beta
• platelet-derived growth factor receptor, beta polypeptide

Background

The APB5 monoclonal antibody reacts with the mouse CD140b molecule, the beta chain of the platelet derived growth factor receptor (PDGF receptor). PDGFRb is a receptor tyrosine kinase that forms dimers on the surface upon ligand binding and phosphorylates substrates. Dimers of PDGFR consist of either homodimers of alpha/alpha, beta/beta, or heterodimers of alpha/beta and serve as a substrate for its kinase activity. CD140b is expressed by embryonic tissues and mesenchymal-derived cells of the adult mouse tissues. The PDGFR beta chain is reported to play a significant role in formation of fibrous atherosclerotic lesions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PDGF R beta Antibody (NBP1-43349)(6)

Publications using NBP1-43349

• Dhumale P, Nielsen J, Hansen A et al. CD31 defines a subpopulation of human adipose-derived regenerative cells with potent angiogenic effects Research Square 2023-05-19
• Dhumale P, Nielsen JV, Hansen ACS et al. CD31 defines a subpopulation of human adipose-derived regenerative cells with potent angiogenic effects Sci Rep 2023-09-01 [PMID: 37658225]
• Zhao Y, Qiao S, Zhang B Et al. A novel neuroinflammation-responsive hydrogel based on mimicking naked mole rat brain microenvironment retards neurovascular dysfunction and cognitive decline in Alzheimer s disease Front Aging Neurosci 2022-07-21 [PMID: 35860673]
• Lechertier T, Reynolds LE, Kim H et al. Pericyte FAK negatively regulates Gas6/Axl signalling to suppress tumour angiogenesis and tumour growth Nat Commun 2020-06-04 [PMID: 32499572] (Protein Array, Mouse)
• Munji RN, Soung AL, Weiner GA et al. Profiling the mouse brain endothelial transcriptome in health and disease models reveals a core blood-brain barrier dysfunction module Nat. Neurosci. 2019-10-14 [PMID: 31611708] (FLOW, Mouse)
• Groman-Lupa S, Adewumi J, Park KU, Brzezinski Iv JA. The Transcription Factor Prdm16 Marks a Single Retinal Ganglion Cell Subtype in the Mouse Retina. Invest. Ophthalmol. Vis. Sci. 2017-10-01 [PMID: 29053761] (IHC-Fr, Mouse)
  
  Details:
  PDGF R beta antibody used as a cell marker to distinguish mural cells from endothelial cells

Review for PDGF R beta Antibody (NBP1-43349) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer Flow Mouse 11/12/2018

Summary

• Application: Flow Cytometry
• Sample Tested: Brain pericytes
• Species: Mouse
• Lot: E04060-1630

Comments

• Comments: Mouse brain pericytes were examined for PDGFRB surface expression

Bioinformatics

• Gene Symbol: PDGFRB
• Entrez:
  • 18596(Mouse)
• Uniprot:
  • P05622()