Recombinant Mouse PDGF-B Protein

Product Discontinued

Recombinant Mouse PDGF-B Protein Summary

• Description: Recombinant bioactive protein containing 109 amino acids for Mouse PDGF-B
  
  

  Source:E. coli
  Amino Acid Sequence:(P31240)

• Details of Functionality: The ED50 as determined by the dose-dependent proliferation of murine BALB/c 3T3 cells is < 2 ng/ml.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: PDGFB
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: <0.1 ng/ug

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 24.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized with no additives.
• Concentration: Lyoph
• Purity: >95%, by SDS-PAGE
• Reconstitution Instructions: Reconstitute in sterile 100 mM acetic acid and 0.1% BSA to 0.1 - 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4C for 1 week or -20C for long-term storage.

Alternate Names for Recombinant Mouse PDGF-B Protein

• B chain
• Becaplermin
• C-Sis
• FLJ12858
• IBGC5
• PDGF subunit B
• PDGF2
• PDGF-2
• PDGFB
• PDGF-B
• platelet-derived growth factor 2
• platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis)oncogene homolog)
• Platelet-derived growth factor beta polypeptide
• platelet-derived growth factor subunit B
• platelet-derived growth factor, B chain
• Platelet-derived growth factor, beta polypeptide (oncogene SIS)
• Proto-oncogene c-Sis
• SIS
• SSV

Background

Recombinant mouse Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle and connective tissue. PDGF, which is composed of a homodimeric, non-glycosylated, polypeptide of 109 amino acids and having a molecular mass of 24.4 kDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: PDGFB