Recombinant Mouse PDGF-B Protein

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications PAGE
Concentration
Lyoph

Order Details

Recombinant Mouse PDGF-B Protein Summary

Description
Recombinant bioactive protein containing 109 amino acids for Mouse PDGF-B

Source:E. coli
Amino Acid Sequence:(P31240)

Details of Functionality
The ED50 as determined by the dose-dependent proliferation of murine BALB/c 3T3 cells is < 2 ng/ml.
Source
E. coli
Protein/Peptide Type
Biologically Active Protein
Gene
PDGFB
Purity
>95%, by SDS-PAGE
Endotoxin Note
<0.1 ng/ug

Applications/Dilutions

Theoretical MW
24.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
Lyophilized with no additives
Concentration
Lyoph
Purity
>95%, by SDS-PAGE
Reconstitution Instructions
Reconstitute in sterile 100mM acetic acid and 0.1% BSA to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4C for 1 week or -20C for future use.

Alternate Names for Recombinant Mouse PDGF-B Protein

  • B chain
  • Becaplermin
  • FLJ12858
  • PDGF subunit B
  • PDGF2
  • PDGF-2
  • PDGFB
  • PDGF-B
  • platelet-derived growth factor 2
  • platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis)oncogene homolog)
  • Platelet-derived growth factor beta polypeptide
  • platelet-derived growth factor subunit B
  • platelet-derived growth factor, B chain
  • Platelet-derived growth factor, beta polypeptide (oncogene SIS)
  • Proto-oncogene c-Sis
  • SSV

Background

Recombinant mouse Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle and connective tissue. PDGF, which is composed of a homodimeric, non-glycosylated, polypeptide of 109 amino acids and having a molecular mass of 24.4 kDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

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Bioinformatics

Gene Symbol PDGFB
Entrez