PDGF-B Antibody (MM0014-5F66)

Images

 
Immunohistochemistry-Paraffin: PDGFB Antibody (MM0014-5F66) [NB110-60980] - Human placenta (formalin fixed and paraffin embedded) section is subjected to IHC.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, IHC-P, CyTOF-ready
Clone
MM0014-5F66
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

PDGF-B Antibody (MM0014-5F66) Summary

Immunogen
This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse immunized with human PDGF BB recombinant protein.
Specificity
This antibody shows approximately some degree (40%) cross-reactivity with rPDGF-AB and no cross-reactivity with recombinant PDGF-AA.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PDGFB
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • CyTOF-ready
Application Notes
Flow Cytometry was reported in scientific literature. This antibody shows approximately some degree (40%) cross-reactivity with rPDGF-AB and no cross-reactivity with recombinant PDGF-AA. Proteinase K (20ug/ml in PBS) is recommended for antigen retrieval. This antibody is CyTOF ready.
Reviewed Applications
Read 1 Review rated 4
using
NB110-60980 in the following applications:

Publications
Read Publication using
NB110-60980 in the following applications:

  • 1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from 0.2 um filtered PBS
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein G purified
Reconstitution Instructions
Reconstitute with sterilized PBS to a final concentration of 0.2 mg/ml.

Alternate Names for PDGF-B Antibody (MM0014-5F66)

  • B chain
  • Becaplermin
  • C-Sis
  • FLJ12858
  • IBGC5
  • PDGF subunit B
  • PDGF2
  • PDGF-2
  • PDGFB
  • PDGF-B
  • platelet-derived growth factor 2
  • platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis)oncogene homolog)
  • Platelet-derived growth factor beta polypeptide
  • platelet-derived growth factor subunit B
  • platelet-derived growth factor, B chain
  • Platelet-derived growth factor, beta polypeptide (oncogene SIS)
  • Proto-oncogene c-Sis
  • SIS
  • SSV

Background

Platelet Derived Growth Factor (PDGF) is the major serum mitogen for cells of mesenchymal origin in humans. The biologically active protein is a dimmer composed of two related polypeptides designated A and B. The PDGF protein has been implicated both directly as well as indirectly in several pathological states including neoplasia, arthritis, arteriosclerosis and bone marrow sclerosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PDGF-B Antibody (NB110-60980)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: FLOW, WB.


Filter By Application
FLOW
(1)
WB
(1)
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Filter By Species
Human
(1)
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Review for PDGF-B Antibody (NB110-60980) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Equine.

Reviews using NB110-60980:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Equine
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin PDGF-B NB110-60980
Enlarge
4
reviewed by:
Anonymous
IHC-P Equine 11/10/2017
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedUterus tissue
SpeciesEquine
LotMM0014-5F66

Comments

CommentsIHC (P) on equine endometrium using the antibody at 1:200 dilution.
Protein is expressed primarily in the luminal epithelium and to a lesser extent in glandular epithelium. No staining on neg. control (omission of the prim AB and replacement with mouse normal serum). Heat-induced antigen retrieval with EDTA based solution for 10 min.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for PDGF-B Antibody (NB110-60980). (Showing 1 - 1 of 1 FAQ).

  1. Does Novus Bio own the cell line for Mab MM0014-5F66 (Cat# NB110-60980)?
    • Unfortunately Novus does not own the cell line for this monoclonal.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB110-60980AF350
Alexa Fluor 405 NB110-60980AF405
Alexa Fluor 488 NB110-60980AF488
Alexa Fluor 532 NB110-60980AF532
Alexa Fluor 594 NB110-60980AF594
Alexa Fluor 647 NB110-60980AF647
Alexa Fluor 700 NB110-60980AF700
Alexa Fluor 750 NB110-60980AF750
Allophycocyanin NB110-60980APC
Biotin NB110-60980B
DyLight 350 NB110-60980UV
DyLight 405 NB110-60980V
DyLight 488 NB110-60980G
DyLight 550 NB110-60980R
DyLight 594 NB110-60980DL594
DyLight 650 NB110-60980C
DyLight 680 NB110-60980FR
DyLight 755 NB110-60980IR
FITC NB110-60980F
HRP NB110-60980H
Janelia Fluor 549 NB110-60980JF549
Janelia Fluor 646 NB110-60980JF646
PE NB110-60980PE
PerCP NB110-60980PCP

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Contact Information

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Anonymous
11/10/2017
Application: IHC-P
Species: Equine

Bioinformatics

Gene Symbol PDGFB
Entrez
Uniprot