PDGF-B Antibody (108128) [Unconjugated]

• Reactivity: Hu
• Applications: WB, ELISA(Cap)
• Clone: 108128
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

PDGF-B Antibody (108128) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human PDGF‑BB
  Ser82-Thr190
  Accession # P01127
• Specificity: Detects the human PDGF-B subunit in direct ELISAs and Western blots. In Western blots, shows 50-100% cross-reactivity with recombinant human (rh) PDGF‑AB and rrPDGF-BB but no cross-reactivity with rhCTGF, rhPlGF, rhVEGF‑B₁₈₆, rhVEGF‑C, or rhVEGF‑D.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PDGFB
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 1 ug/mL
• Application Notes: ELISA Capture: Human PDGF-B Antibody (Catalog # MAB1739)
  ELISA Detection: Human PDGF-AA/AB Biotinylated Antibody (Catalog # BAF221)
  Standard: Recombinant Human PDGF-AB (Catalog # 222-AB)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PDGF-B Antibody (108128) [Unconjugated]

• B chain
• Becaplermin
• C-Sis
• FLJ12858
• IBGC5
• PDGF subunit B
• PDGF2
• PDGF-2
• PDGFB
• PDGF-B
• platelet-derived growth factor 2
• platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis)oncogene homolog)
• Platelet-derived growth factor beta polypeptide
• platelet-derived growth factor subunit B
• platelet-derived growth factor, B chain
• Platelet-derived growth factor, beta polypeptide (oncogene SIS)
• Proto-oncogene c-Sis
• SIS
• SSV

Background

Four PDGF family members, A, B, C and D, have been identified. PDGF-B can exist as a disulfide-linked homodimer (PDGF-BB) or heterodimer (PDGF-AB). PDGF-B binds and activates PDGF R alpha and PDGF R beta .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PDGF-B Antibody (MAB1739)(2)

Publications using MAB1739

• Oana Mihaela Tudoran, Olga Soritau, Loredana Balacescu, Laura Pop, Guillaume Meurice, Simona Visan et al. PDGF beta targeting in cervical cancer cells suggest a fine-tuning of compensatory signalling pathways to sustain tumourigenic stimulation Journal of Cellular and Molecular Medicine 2015-02-01 [PMID: 25311137]
• Oana Tudoran, Olga Soritau, Ovidiu Balacescu, Loredana Balacescu, Cornelia Braicu, Meda Rus et al. Early transcriptional pattern of angiogenesis induced by EGCG treatment in cervical tumour cells Journal of Cellular and Molecular Medicine 2012-03-01 [PMID: 21609393]

Bioinformatics

• Gene Symbol: PDGFB
• Uniprot:
  • P01127()