PD-L2/B7-DC/PDCD1LG2 Antibody

PD-L2 was detected in perfusion fixed frozen sections of mouse thymus using 15 µg/mL Goat Anti-Mouse PD-L2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1022) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: WB, Flow, IHC, B/N, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

PD-L2/B7-DC/PDCD1LG2 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse PD-L2
  Leu20-Arg219
  Accession # Q9WUL5
• Specificity: Detects mouse PD-L2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant human PD-L2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PDCD1LG2
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PD-L2/B7-DC/PDCD1LG2 Antibody

• B7-DC
• bA574F11.2
• Btdc
• Butyrophilin B7-DC
• Butyrophilin-like Protein
• CD273 antigen
• CD273
• CD273PD-1 ligand 2
• MGC142240
• PD-1-ligand 2
• PDCD1L2MGC142238
• PDCD1LG2
• PDL2
• PD-L2
• PDL2B7DC
• PD-L2PDCD1 ligand 2
• programmed cell death 1 ligand 2
• Programmed death ligand 2

Background

Mouse Programmed Death Ligand 2 (PD-L2), also named B7DC and butyrophilin-like protein, is a member of the B7 family of proteins that provide signals for regulating T-cell activation and tolerance (1-4). Other family members include B7-1, B7-2, B7-H2, PD-L1 (B7-H1), and B7-H3. B7 proteins are immunoglobulin (Ig) superfamily members with extracellular Ig-V-like and Ig-C-like domains and short cytoplasmic domains. Among the family members, they share from 20-40% amino acid (aa) sequence identity. The cloned mouse PD-L2 cDNA encodes a 247 aa type I membrane precursor protein with a putative 20 aa signal peptide, a 199 aa extracellular region containing one V-like and one C-like Ig domain, a 23 aa transmembrane region, and only a 5 aa cytoplasmic domain. The extracellular domains of mouse and human PD-L2 share approximately 72% aa sequence identity. PD-L2 is one of two ligands for programmed death-1 (PD-1), a member of the CD28 family of immunoreceptors. The other identified ligand is PD-L1. Mouse PD-L1 and PD-L2 share approximately 34% aa sequence identity and have similar functions. PD-L2 is constitutively expressed in lymphoid and non-lymphoid organs (1-4). The expression of PD-L2 is detected on dendritic cells, thymic epithelial cells and IFN-gamma treated monocytes. PD-L2 expression is also upregulated in a variety of tumor cell lines. On previously activated T cells, PD-L2 interaction with PD-1 inhibits TCR-mediated proliferation and cytokine production, suggesting an inhibitory role in regulating immune responses. In contrast, a co-stimulatory function for the PD-1 ligands on resting T cells has also been reported.

1. Latchman, Y. et al. (2001) Nature Immun. 2:261.
2. Tseng, B.S-Y. et al. (2001) J. Exp. Med. 193:839.
3. Sharpe, A.H. and G.J. Freeman (2002) Nat. Rev. Immunol. 2:116.
4. Coyle, A. and J. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PD-L2/B7-DC/PDCD1LG2 Antibody (AF1022)(5)

Publications using AF1022

• W Deng, S Zhu, L Zeng, J Liu, R Kang, M Yang, L Cao, H Wang, TR Billiar, J Jiang, M Xie, D Tang The Circadian Clock Controls Immune Checkpoint Pathway in Sepsis Cell Rep, 2018-07-10;24(2):366-378. 2018-07-10 [PMID: 29996098] (Western Blot, Mouse)
• Guoning Guo, Hong Li, Dayan Cao, Yongwen Chen The development of endometrial hyperplasia in aged PD-1-deficient female mice Diagnostic Pathology 12/1/2014 [PMID: 24886298]
• Yu Zhao, GanLan Bian, CaiYong Yu, FangFang Liu, Ling Liu, HongMin Guo et al. Cross-reactivity of anti-programmed death ligand 2 polyclonal antibody in mouse tissues Science China Life Sciences 11/1/2012 [PMID: 23090063]
• Kasagi S, Kawano S, Okazaki T, Honjo T, Morinobu A, Hatachi S, Shimatani K, Tanaka Y, Minato N, Kumagai S Anti-programmed cell death 1 antibody reduces CD4+PD-1+ T cells and relieves the lupus-like nephritis of NZB/W F1 mice. J. Immunol., 2010-02-05;184(5):2337-47. 2010-02-05 [PMID: 20139271] (IHC-Fr, Mouse)
• Wang J, Yoshida T, Nakaki F, Hiai H, Okazaki T, Honjo T Establishment of NOD-Pdcd1-/- mice as an efficient animal model of type I diabetes. Proc. Natl. Acad. Sci. U.S.A., 2005-08-08;102(33):11823-8. 2005-08-08 [PMID: 16087865] (ICC, Mouse)

Review for PD-L2/B7-DC/PDCD1LG2 Antibody (AF1022) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer IF Mouse 01/05/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 23090063
• Species: Mouse

Bioinformatics

• Gene Symbol: PDCD1LG2
• Uniprot:
  • Q9WUL5()