PD-L2/B7-DC/PDCD1LG2 Antibody (Z64P2D3*H4) - BSA Free

Immunohistochemistry-Paraffin: PD-L2/B7-DC/PDCD1LG2 Antibody (Z64P2D3-H4) [NBP2-52408] - Staining was performed on formalin-fixed, paraffin-embedded human testis seminoma tissue sections using anti-PDL2 (soluble form) [Z64P2D3*H4]. Cytoplasmic staining was observed.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: Z64P2D3*H4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

PD-L2/B7-DC/PDCD1LG2 Antibody (Z64P2D3*H4) - BSA Free Summary

• Immunogen: Ovalbumin-conjugated synthetic peptide KAVFFKRHN
• Clonality: Monoclonal
• Host: Mouse
• Gene: PDCD1LG2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100 - 1:2000
• Application Notes: For IHC, perform antigen retrieval step: microwave 20 min @ 800W in 10 mM citrate buffer, pH 6.0. Positive control(s): IHC: formalin-fixed, paraffin-embedded multi tumour tissue microarray.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for PD-L2/B7-DC/PDCD1LG2 Antibody (Z64P2D3*H4) - BSA Free

• B7-DC
• bA574F11.2
• Btdc
• Butyrophilin B7-DC
• Butyrophilin-like Protein
• CD273 antigen
• CD273
• CD273PD-1 ligand 2
• MGC142240
• PD-1-ligand 2
• PDCD1L2MGC142238
• PDCD1LG2
• PDL2
• PD-L2
• PDL2B7DC
• PD-L2PDCD1 ligand 2
• programmed cell death 1 ligand 2
• Programmed death ligand 2

Background

B7-DC is also called programmed death ligand 2 (PDL2). It has recently been clustered as CD273. This ligand is a 42 kD member of the immunoglobulin receptor superfamily expressed on a subset of dendritic cells, liver and a small subset of macrophages as well as a few transformed cell lines. B7-DC (CD273) has been reported to be stimulatory on dendritic cells when cross-linked and to inhibit T cell activation upon engaging the PD-1 receptor. B7-DC (CD273) has also been reported to bind to an alternative receptor and to mediate T cell activation through such non-PD1 mediated interactions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PDCD1LG2